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11. TOXICOLOGICAL INFORMATION <br />Skin: The LD50 for skin absorption in rabbits is >10,000 mg/kg. <br />Ingestion: The oral LD50 for rats is 20,000-34,000 mg/kg. <br />Mutagenicity: In vitro mutagenicity studies were negative. Animal mutagenicity studies were negative. <br />12. ECOLOGICAL INFORMATION <br />Movement & Partitioning: Based largely or completely on information for similar material(s), i.e., propylene <br />glycol. Bioconcentration potential is low (BCF less than 100 or Log Pow less than 3). Log octanol / water partition <br />coefficient (log Pow) is —0.92. Henry Law Constant (H) is 1.2E-8 atm.m3/mole. <br />Degradation & Transformation: Based largely or completely on information for similar material(s) i.e., <br />propylene glycol. Biodegradation under aerobic static laboratory conditions is high (BOD20 OR BOD28/ThOD <br />greater than 40%). Biodegradation is expected to be achievable in a secondary wastewater treatment plant. 5 -day <br />biochemical oxygen demand (BODS) is 1.16 p/p. 200 -day biochemical oxygen demand (BOD20) is 1.45 p/p. <br />theoretical oxygen demand (THOD) is calculated to be 1.68 p/p. Inhibitory concentration (IC50) in OECD <br />Activated Sludge Respiration Inhibition Test (OECD Test No. 209) is greater than 1 gm/L. Degradation is expected <br />in the atmospheric environment within minutes to hours. <br />Ecotoxicology: Based largely or completely on information for similar material (s), i.e., propylene glycol. Material <br />is practically non-toxic to aquatic organisms on an acute basis (LC50 greater than 100 mg/L in most sensitive <br />species). <br />Acute LC50 for fathead minnow (Pimephales promelas) is 4,600-54900 mg/L <br />Acute LC50 for guppy (Poecilia reticulata) is greater than 10000 mg/L. <br />Acute LC50 for water flea Daphnia magna is 4850-34400 mg/L <br />Acute LC50 for rainbow trout (Oncorhynchus mykiss) is 44mL/L (about 44000 mg/L) <br />13. DISPOSAL CONSIDERATIONS <br />Waste Disposal Method; Landfill solids at permitted sites using registered transporters. Burn concentrated <br />liquids, avoiding flameouts and assuring emissions comply with applicable regulations. Diluted aqueous waste may <br />biodegrade, but avoid overloading plant biomass and assure effluent complies with applicable regulations. <br />4 <br />S. Bravo Systems, Inc. Interstitial Fluid <br />