Laserfiche WebLink
2 <br /> Percentage of Strain T es in Each Sam le: <br /> Samples MW-9 MW-12 MW-13 Description <br /> Strain .� <br /> 1 60% _ 40% Med, tan, shiny <br /> 2 _ 100% 30% Med, white, shiny <br /> 3 40% _ _ large,pale yellow <br /> 4 _ _ 30% White,mucosal <br /> GC-FAME & Bioloe Processing: <br /> Following isolation the strams were mdividually streaked onto TSA. The TSA plates were <br /> processed after 24 hour incubation by [Method 1 - Standard GC-FAME]. The strains were <br /> examined against both the Aerobe (TSBA[rev 3.80]) and Clinical Aerobe (CLIN[rev.3.80])GC- <br /> FAME databases. Subsequently the strams were prepared for BiologTM analysis by suspending <br /> them in sterile salute and loachng the solutions into the appropriate microtiter plates (Gram negative <br /> or Gram positive) The plates were incubated for 24 hours and then examined against version 3 5 <br /> of the BiologTM database using an automated microplate reader. <br /> Summary of GC-FAMEBiolog Analyses <br /> Strain Primary Sim. Dist Primary ID by Plate Sim. Dist. <br /> Name Identification by Coef. Coef. BiologTM Type Coef Coef <br /> 2600-1 Pseudomonas 0.625 3.135 Pseudomonas GN 0 973 0 288 <br /> chlororaphis fluorescens type A <br /> 2600-2 Acmetobacterjohnsonu 0 194 585 Acmetobacter GN 0 674 3.946 <br /> ahnsorarlgenospecies 7 <br /> 2600-3 Pseudomonas synagae 0.355 4 654 Pseudomonas GN 088 1679 <br /> syrmgae fluorescens type A <br /> 2600.4 Pseudomonas 0.238 5 473 Pseudomonas GN 0 632 4 746 <br /> savastanoc pv frannus fluorescens type G <br /> 1 Similarity and Distance Coefficient <br /> In order to create the database that we use to identify your organisms, thousands of species of <br /> bacteria had to be tested.in fact each species itself had to be tested hundreds of times to determine <br /> I a set of characteristics unique to it The species characteristics that are in our database are an <br /> "average"of the characteristics of hundreds of tested bactena of the same species.The Similarity <br /> and Distance Coefficient of your organism refers to the similarity and distance to the hypothetical <br /> ' `mean' organism in the database. The database organism has a similarity coefficient of one and a <br /> distance of zero So the closer your strum is to one and zero the more closely it matches the mean <br /> organism in the database <br /> A good match is one with a similarity coefficient greater than.5 and a distance coefficient of less <br /> than 7. <br />