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Pacific EcoRisk Environmental Consulting&Testing <br /> 1.INTRODUCTION <br /> Under contract to GeoAnalytical Laboratories,Inc.(GeoAnalytical),Pacific EcoRisk(PER) <br /> conducted a screening Title 22"Hazardous Waste"fish toxicity evaluation of a waste sample, <br /> designated "Tire Fire Sample'.This toxicity test consisted of a 96-hr static bioassay with <br /> juvenile fathead minnows (Pimephales promelas). This report describes the performance and <br /> results of this test. <br /> 2.ACUTE TOXICITY TEST PROCEDURES <br /> The methods used in conducting this test followed the guidelines established by the California <br /> Dept.of Fish &Game manual "Static Acute Bioassay Procedures for Hazardous Waste Samples" <br /> (Polisini and Miller 1988),with additional guidelines as provided by the California Dept.of <br /> Health Services(Janna White,personal communication). <br /> 2.1 Receipt and Handling of the Waste Sample <br /> A waste sample,designated"Tire Fire Sample",was delivered to the PER testing laboratory in <br /> Fairfield on May 6,2009,where it was placed under refrigeration at 4'C until being used to <br /> prepare the test solutions. The chain-of-custody record for the collection and delivery of this <br /> waste sample is provided in Appendix A. <br /> 2.2 Test Organisms <br /> The Pimephales promelas used in this test were obtained from a commercial supplier(Thomas <br /> Fish Company,Anderson,CA).These fish were maintained at 20'C in aerated aquaria <br /> containing EPA synthetic soft water prior to their use in this test. During this pre-test period,the <br /> fish were fed brine shrimp nauplii ad libitum. <br /> 23 Preparation of the Test Media <br /> Sub-samples of the waste were mixed with the Lab Control/dilution water for 6 his,using a <br /> rotary mixer.The Lab Control/dilution water consisted of synthetic US EPA "soft" water <br /> (prepared by adding reagent grade salts to reverse-osmosis de-ionized water).After mixing,these <br /> preliminary solutions were used to prepare test solutions at sample concentrations of 250,500, <br /> and 750 mg/L. <br /> 2.4 Acute Toxicity Testing with Fathead Minnows <br /> This hazardous waste evaluation was run at sample concentrations of 250,500,and 750 mg/L, <br /> with a Lab Control consisting of the same synthetic EPA "soft" water.Water quality <br /> Page I �! Pacific EcoRisk tii> <br /> 5114 <br />