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n Pacific EcoRisk Environmental Consulting&Testing characteristics(pH and dissolved oxygen ID.O.1) were determined for the test solution in each test replicate prior to initiation of this test.There were 2 replicates at each test treatment,each replicate consisting of 9.2 L of media in a 10-L container.The test was initiated with the random allocation of I0 juvenile fathead minnows into each replicate container. These containers were placed in a temperature-controlled room at 20°C,under fluorescent lighting on a 16L:8D photoperiod. Each day,each replicate container was examined,and the number of live fish in each was recorded.Routine water quality characteristics(temperature,pH,and D.O.)of the treatment waters were measured and recorded for each replicate container each day. After 96(t-2) hrs,the test was terminated and the number of live fish in each replicate container was determined.The fish in a randomly selected Lab Control treatment replicate were then euthanized and measured for total length(to the nearest 0.1 mm)and weighed (to the nearest 0.01 g).The resulting survival data for the waste treatments were evaluated to determine the LC50 using the CETIS®statistical software (TidePool Scientific,McKinleyville,CA). 3.RESULTS 3.1 Effects of the Waste Sample on Fathead Minnows The results of this test are summarized in Table 1.There was 100%survival at the Lab Control treatment;there was 95% survival in each of the waste treatment levels.The LC50 of this sample is greater than the"hazardous waste" toxicity threshold of 500 mglL,indicating that this sample should 01 be considered "hazardous" with respect to fish toxicity. The test data and summary of statistics for this test are presented in Appendix B. Table 1.Results of the hazardous waste toxicity evaluation of the waste sample"Tire Fire Sample". Sample Treatment Mean % Survival Lab Control 100 250 mglL 95 500 mg/L 95 750 mglL 95 LC5o=>750 mg/L - -- Page 2 Pacific EeoRisk t > siva