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Pacific EcoRisk Environmental Consulting & Testing 1. INTRODUCTION Under contract to GeoAnalytical Laboratories, Inc. (GeoAnalytical), Pacific EcoRisk (PER) conducted a screening level Title 22 "Hazardous Waste" fish toxicity evaluation of a waste sample, designated "Sample 1 A". This toxicity test consisted of a 96 -hr static bioassay with juvenile fathead minnows (Pimephales promelas). This report describes the performance and results of this test. 2. ACUTE TOXICITY TEST PROCEDURES The methods used in conducting this test followed the guidelines established by the California Dept. of Fish & Game manual "Static Acute Bioassay Procedures for Hazardous Waste Samples" (Polisini and Miller 198$), with additional guidelines as provided by the California Dept. of Health Services (Janna White, personal communication). 2.1 Receipt and Handling of the Waste Sample A waste sample, designated "Sample IA", was delivered to the PER testing laboratory in Fairfield on October 22, 2010, where it was placed under refrigeration at 4'C until being used to prepare the test solutions. The chain -of -custody record for the collection and delivery of this waste sample is provided in Appendix A. 2.2 Test Organisms The Pimephales promelas used in this test were obtained from a commercial supplier (Thomas Fish Company, Anderson, CA). These fish were maintained at 20`C in aerated aquaria containing EPA synthetic soft water prior to their use in this test. 2.3 Preparation of the Test Media Sub -samples of the waste were mixed with the Lab Control/dilution water for 6 hrs, using a rotary mixer. The Lab Control/dilution water consisted of synthetic US EPA "soft" water (prepared by adding reagent grade salts to Type 1 l lab water [reverse -osmosis de -ionized water]). After mixing, these preliminary solutions were used to prepare test solutions at sample concentrations of 250, 500, and 750 mg/L. Page 1� 4112