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OCT -96-2006 09:32AM FROM -EVERGREEN CIMTOiER SERVICE NORTH <br />Jul 13 20t1G 1:topm I LASERJET FRX <br />+510 744 9880 T-636 P. 005/011 F-992 <br />_ P-2 <br />SES <br />1. INTRODUCTION <br />The Califomin Deportment of Substances Control has adopted regulations (R-45-78) which <br />define criteria for the identification of hazardous wastes. These criteria are codified in Chapter <br />11, Article 3 of Title 22 of the California Code of Regulations. Toxicity to aquatic life, <br />specifically fish, is one of the criteria used to gauge the hazardous pcINential of a waste. An acute <br />96 -hour bioassay is used to determine the TrC50 as defined in Section 66261.24(a)(6) of these <br />regulations. This 96 -hour LC50 value serves as the nuncrical indicator of the toxicity of a waste <br />to aquatic life. The sample is deemed hazardous if the LC50 is less then $00 mg/L. <br />This report deszibw the procedum used and the results obtained for the hazardous waste <br />aquatic toxicity scrctxting test(s) performed by Block Environmen►al Services (BES) for <br />McCamphell Analytical. <br />BES is an Environmental Laboratory Accreditation Program certified laboratory (#1812). <br />2. MATERIALS AND MXdTHDDS <br />2.1 TEST ORGANISMS <br />• Rainbow Trout, Oncorhynchus myldss, obtained from a commercial supplier. <br />2.2 TEST PROCEDURES <br />A detailed procedure for this test is outlined in labomtoty standard operating procedures (SOPS) <br />kept at the BES laboratory. Thesc Sops are based upon the fallowh3g references: <br />California's Title 22 Code, Section N6261.24(a)(6); StaLrk� Acute Bleassay <br />Prooedurer for Razardor s Waste Somples, Polisini and MiUeT, 1999, California <br />Department of Piste and Raine <br />Guideltnes.for Performing Static Acute Toxicity Fish Bioassays in Municipal and <br />Industrial Wastewaters, Koppardahl, 1976, California bapattmect of Fish and Game <br />Standard Methods for the Examination of Water and Wpstewater, 19's Edition, <br />American Public Kcalth Association, 1993. <br />2.3 DATA ANALYSIS <br />All toxicity testing results will be analyzed wing an appropriate statistical method to determine a <br />LC50 and the corresponding 950A confidence limits. <br />BES #23364 Page 2 of 3 <br />