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0 0 <br /> Pacific EcoRisk Environmental Consulting&Testing <br /> 2.4 Acute Toxicity Testing with Fathead Minnows <br /> This hazardous waste evaluation was run at sample concentrations of 250,500,and 750 mg/L. <br /> with a Lab Control consisting of the same synthetic EPA"soft" water. Water quality <br /> characteristics (pH and dissolved oxygen ID.O.p were determined for the test solution in each <br /> test replicate prior to initiation of this test.There were 2 replicates at each test treatment,each <br /> replicate consisting of 9.2 L of media in a 10-L plastic container.The test was initiated with the <br /> random allocation of 10 juvenile fathead minnows into each replicate container. These <br /> containers were placed in a temperature-controlled room at 20°C,under fluorescent lighting on a <br /> 16L:8D photoperiod. <br /> Each day,each replicate container was examined,and the number of live fish in each was <br /> recorded. Routine water quality characteristics(temperature,pH,and D.O.) of the treatment <br /> waters were measured and recorded for each replicate container each day. <br /> After 96(t2) hrs,the test was terminated and the number of live fish in each replicate container <br /> was determined.The fish in a randomly selected Lab Control treatment replicate were then <br /> euthanized and measured for total length(to the nearest 0.1 mm) and weighed (to the nearest <br /> 0.01 g).The resulting survival data for the waste treatments were evaluated to determine the <br /> LCso using the CMS'statistical software (Version 1.6.4J,TidePool Scientific, McKinleyville, <br /> CA). <br /> 3.RESULTS <br /> 3.1 Effects of the Waste Sample on Fathead Minnows <br /> The results of this test are summarized in Table 1.There was 100% survival at the Lab Control <br /> treatment; there was 100% survival in each of the waste treatment levels.These results indicate <br /> that the LCso of this sample is greater than the "hazardous waste" toxicity threshold of 500 mg/L, <br /> and that this sample should not be considered "hazardous" with respect to fish toxicity. <br /> The test data and summary of statistics for this test are presented in Appendix B. <br /> Table I.Results of the hazardous waste toxicity evaluation of the waste sample"41008-01". <br /> Sample Treatment Mean % Survival <br /> Lab Control 100 <br /> 250 mg/L 100 <br /> 500 mg/L 100 <br /> 750 mg/L too <br /> LC50= >500 mg/L <br /> Page 2 Pacific EcoRisk I��� <br />