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0 <br /> Pacific EeoRisk Environmental Consulting &Testing <br /> 2.4 Acute Toxicity Testing with Fathead Minnows <br /> This hazardous waste evaluation was run at sample concentrations of 250,500,and 750 mg/L, <br /> with a Lab Control consisting of the same synthetic EPA "soft" water. Water quality <br /> characteristics (pH and dissolved oxygen [D.O.1) were determined for the test solution in each <br /> test replicate prior to initiation of this test.There were 2 replicates at each test treatment,each <br /> replicate consisting of 9.2 L of media in a 10-L plastic container.The test was initiated with the <br /> random allocation of 10 juvenile fathead minnows into each replicate container. These <br /> containers were placed in a temperature-controlled room at 20°C,under fluorescent lighting on a <br /> 16L.8D photoperiod. <br /> Each day, each replicate container was examined,and the number of live fish in each was <br /> recorded. Routine water quality characteristics (temperature, pH,and D.O.) of the treatment <br /> waters were measured and recorded for each replicate container each day. <br /> After 96(t2) firs, the test was terminated and the number of live fish in each replicate container <br /> was determined.The fish in a randomly selected Lab Control treatment replicate were then <br /> euthanized and measured for total length (to the nearest 0.1 mm) and weighed (to the nearest <br /> 0.0I g).The resulting survival data for the waste treatments were evaluated to determine the <br /> LCso using the CETIS'x'statistical software (Version 1.6.41,TidePool Scientific, McKinleyville, <br /> CA). <br /> 3. RESULTS <br /> 3.1 Ef'f'ects of the Waste Sample on Fathead Minnows <br /> The results of this test are summarized in Table I.There was 95% survival at the Lab Control <br /> treatment; there was>95% survival in each of the waste treatment levels.These results indicate <br /> that the LCso of this sample is greater than the"hazardous waste" toxicity threshold of 500 mg/L, <br /> and that this sample should not be considered "hazardous" with respect to fish toxicity. <br /> The test data and summary of statistics for this test are presented in Appendix B. <br /> Table I. Results of the hazardous waste toxicity evaluation of the waste sample "Sioan 0.". <br /> Sample Treatment "dean % Survival <br /> Lab Control 95 <br /> 250 mg/L 95 <br /> 500 mg/1_ 95 <br /> 750 mg/L 100 <br /> LC50= >500 mg/L <br /> Page 2 <br />