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0 <br /> Pacific EcoRisk Environmental Consulting&Testing <br /> 1. INTRODUCTION <br /> Under contract to GeoAnalytical Laboratories, Inc. (GeoAnalytical), Pacific EcoRisk(PER) <br /> conducted a screening Title 22"Hazardous Waste" Fish toxicity evaluation of a waste sample, <br /> designated "Sample 1 West& 2 East". This toxicity test consisted of a 96-hr static bioassay with <br /> juvenile fathead minnows (Pimephales promelas). This report describes the performance and <br /> results of this test. <br /> 2. ACUTE TOXICITY TEST PROCEDURES <br /> The methods used in conducting this test followed the guidelines established by the California <br /> Dept of Fish & Game manual "Static Acute Bioassay Procedures for Hazardous Waste Samples" <br /> (Polisini and Miller 1988), with additional guidelines as provided by the California Dept of <br /> Health Services (Janna White, personal communication). <br /> 2.1 Receipt and Handling of the Waste Sample <br /> A waste sample, designated "Sample 1 West& 2 East", was delivered to the PER testing <br /> laboratory in Fairfield on March 21, 2008,where it was placed under refrigeration at 4°C until <br /> being used to prepare the test solutions. The chain-of-custody record for the collection and <br /> delivery of this waste sample is provided in Appendix A. <br /> 2.2 Test Organisms <br /> The Pimephales promelas used in this test were obtained from a commercial supplier(Thomas <br /> Fish Company, Anderson, CA). These fish were maintained at 20'C in aerated aquaria <br /> containing EPA synthetic soft water prior to their use in this test. During this pre-test period, the <br /> fish were fed brine shrimp nauplii ad libitum. <br /> 2.3 Preparation of the Test Media <br /> Sub-samples of the waste were mixed with the Lab Controlldilution water for 6 hrs, using a <br /> rotary mixer. The Lab Controlldilution water consisted of synthetic US EPA "soft"water <br /> (prepared by adding reagent grade salts to reverse-osmosis de-ionized water). After mixing, these <br /> preliminary solutions were used to prepare test solutions at sample concentrations of 250, 500, <br /> and 750 mg/L. <br /> Page 1 <br />