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Pacific EcoRisk Environmental Consulting &Testing <br /> 1.INTRODUCTION <br /> Under contract to Delta Environmental Laboratories. Pacific EcoRisk (PER)conducted a <br /> screening level Title 22 "Hazardous Waste" fish toxicity evaluation of a liquid waste sample <br /> designated"22029-1".This toxicity test consisted of a 96-hr static bioassay with juvenile fathead <br /> minnows(Pimephales promelas).This report describes the performance and results of this test. <br /> 2.ACUTE TOXICITY TEST PROCEDURES <br /> The methods used in conducting this test followed the guidelines established by the California <br /> Dept. of Fish &Game manual "Static Acute Bioassay Procedures for Hazardous Waste Samples" <br /> (Polisini and Miller 1988),with additional guidelines as provided by the California Dept.of <br /> Health Services (Janna White,personal communication). <br /> 2.1 Receipt and Handling of the Waste Sample <br /> A liquid waste sample designated "22029-1" was delivered to the PER testing laboratory on <br /> March 27,2014,where it was placed under refrigeration at 4°C until being used to prepare the <br /> test solutions.The chain-of-custody record for the collection and delivery of this waste sample is <br /> provided in Appendix A. <br /> 22 Preparation of the Test Media <br /> The waste sample was homogenized,after which sub-samples of the waste were mixed with the <br /> Lab Water Control medium for 6 hrs,using a rotary mixer.The Lab Water Control medium <br /> consisted of synthetic US EPA "soft" water(prepared by adding reagent grade salts to Type I lab <br /> water [reverse-osmosis de-ionized water]). After mixing,these preliminary solutions were used <br /> to prepare test solutions at sample concentrations of 250,500,and 750 mg/L. <br /> 23 Acute Toxicity Testing with Fathead Minnows <br /> The fathead minnows used in this test were obtained from a commercial supplier(Thomas Fish <br /> Company,Anderson,CA).These fish were maintained at 20`C in aerated aquaria containing <br /> EPA synthetic soft water prior to their use in this test. <br /> This hazardous waste evaluation was run at sample concentrations of 250,500,and 750 mgfL. <br /> with a Lab Control consisting of the same synthetic EPA"soft" water.There were 2 replicates at <br /> each test treatment,each replicate consisting of 9.2 L of media in a 10-L container. Water quality <br /> characteristics(pH and dissolved oxygen [D.O.1) were determined for the test solution in each <br /> test replicate prior to initiation of this test. <br /> Page 1 L <br /> uta <br />