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FATHEAD MINNOW HAZARDOUS WASTE <br />SCREEN BIOASSAY <br />Lab No.: AIZC, a1 v/ -v-( <br />iieat/LD: Ate r 12 o Sr�vU c1�- OL;1 <br />TEST SUMMARY <br />Species: Pimephales promelas. <br />Fish weight (gm): av: 0.17 ; min: o.Z ; max: 0.3 y . <br />Reference Toxicant: SDS conducted monthly. <br />Test chamber volume: 10 liters. <br />Temperature: 20 +/- 2°C. <br />Aeration: none, unless D.O. drops below 5.0 mg/I. <br />Number of replicates: 2. <br />Dilution water: Soft reconstituted water (40-48 mg/l CaCO,) <br />TEST DATA <br />Pt I Ge"%Zzw <br />N�fl� TL Aquatic 201¢ <br />Testing <br />QC <br />. Laboratories <br />Source: In -Lab Culture. <br />Regulations: CCR Title 22. <br />Test Protocol: California F&G/DHS 1988. <br />Endpoints: Survival at 96 hrs. <br />Test type: Static. <br />Feeding: None. <br />Number offish per chamber: 10. <br />Photoperiod: 16/8 hrs light/dark. <br />Total Number Dead <br />INITIAL <br />24 Hr <br />Alkalinity Hardness <br />48 Hr <br />IIIIt1aI <br />Final <br />72 Hr <br />96 Hr <br />S -3 `11 (o". <br />Oo <br />5-5-11 <br />110V <br />5 - 6 - / 3 <br />(1 o <br />t Z - I GAG I <br />Date/Time: <br />°C O pH <br />°C DO pH #D <br />°C <br />DO <br />pH # D <br />__._. <br />Analyst: <br />°C <br />DO <br />pH <br /># D <br />°C 0 pH <br /># D <br />1 Za. <br />g. 7 <br />7 b <br />Control A <br />(q <br />3 <br />7.7 <br />177 <br />0 <br />Control B <br />(1,7 <br />S, <br />7 g <br />1.5 <br />g.7 <br />7.7 <br />7 7 <br />77 <br />tid <br />400 mg/1 A <br />ts, <br />$. 7 <br />7.8 <br />S <br />S. 7 <br />7. <br />�. <br />8. 7 <br />7- 7 <br />�I.6 <br />g 6 <br />7,7 <br />c��f <br />��� O, 7 - <br />400 mg/1 B <br />g g <br />7.8 <br />1111-17 <br />Q. i <br />7$ <br />v <br />• L <br />s <br />7, 7 <br />?o• 6 <br />S, S <br />7 7 <br />U <br />U� <br />750 mg/I A <br />Iy. y <br />-a <br />1. 0 <br />�•`i <br />7 `C <br />6 <br />Ze.�( <br />7.0 <br />G <br />?o. j <br />3• <br />7, <br />750 mg/l B <br />11.7 q'$ 17.1 Z�, y g .S- 7. $ <br />i,. y <br />�•s' <br />7. it 0 <br />?.. 6 <br />f. Y <br />1 7.2 d <br />Comments: <br />Extraction method: Mechanical shaking <br />_ <br />c <br />None (aqueous solution) <br />Dissolved Oxygen (DO) readings in mg/l 02. Test Aerated: V�p/ No <br />Total Number Dead <br />CONTROL <br />RIGH CONCENTRATION <br />Alkalinity Hardness <br />Alkalinity Hardness <br />IIIIt1aI <br />Final <br />3,Z mg/I CaCO, Z(t. mg/I CaCO, <br />LI mg/1 CaCO, t{ S mg/1 CaCO, <br />mgA CaCO, 6 mg/1 CaCO, <br />3 mg/1 CaCO, f,, mgA CaCO, <br />Total Number Dead <br />Control <br />/20 <br />400 mg/I <br />/20 <br />750 m I <br />/20 <br />RESULTS <br />the checked result -applies based on fish survival rates <br />PASSED LC50 > 750 mg/1 (<40% dead in 750 mg/I cone.) <br />FAILED 240% dead in 750 mg/I (close to passing -definitive test recommended) <br />FAILED LC50 < 400 mg/I (>60% dead in 400 mg/I cone.) <br />