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************************************************************************* <br />STUDY NO.: <br />23E-93 <br />95 Percent Confidence Limits <br />ASSAY START <br />11/01/93 <br />SPECIES: <br />Pimephales <br />promelas <br />EXPOSURE: <br />96 hours <br />'PLE: SDS Ref Tcx Test SOFT Batch 090893 Lab <br />The binomial test shows that 12.5 and 50 can be used <br />as statistically sound, conservative 95 percent confidence limits <br />because the actual confidence level associated with these limits <br />is greater than 95 percent. <br />25 can be used as an approximate LC50 for this set of data. <br />-----------RESULTS CALCULATED USING THE MOVING AVERAGE METHOD ---------- <br />Span G <br />LC50 <br />95 Percent Confidence Limits <br />3 6.572934E-02 <br />22.87834 <br />18.74524 <br />28.95456 <br />2 .1198137 <br />23.54723 <br />18.69278 <br />29.18284 <br />1 .3484681 <br />25 <br />13.87341 <br />31.09513 <br />1 .7002976 <br />25 <br />18.64277 <br />169.3438 <br />An LC50 calculated using <br />the Moving <br />Average method may <br />not be a very good <br />estimate if the span is much <br />less than the number of <br />concentrations. <br />-------------- RESULTS CALCULATED <br />USING THE PROBIT MET:HOD--------------- <br />Iterations G <br />H <br />Chi -Square <br />Probability <br />5 .1324919 <br />1 <br />.7273731 <br />.8667473 <br />3' ne = 4,477848 <br />LC50 = 23.82512 <br />%,� 95 Percent Confidence <br />Limits = 2.847937 and <br />6.10776 <br />__C50 = 23.23474 <br />Lower: 19.06122 <br />95 Percent Confidence <br />Limits = <br />18.88599 and <br />28.80504 <br />---------RESULTS CALCULATED USING THE TRIMMED SPEARMAN -BARBER METHOD <br />Dose <br />Percent <br />Monotonic <br />Trimmed <br />:onc. =Ln (Conc) <br />Dead <br />Rel. Freq. <br />Rel. Freq. <br />50 3.912023 <br />95 <br />.95 <br />1.0625 <br />25 3.218876 <br />50 <br />.5 <br />.5 <br />12.5 2.525729 <br />15 <br />.15 <br />6.250001E-02 <br />6.25 1.832581 <br />0 <br />0 <br />-.125 * <br />3.1 1.131402 <br />0 <br />0 <br />-.125 * <br />Alpha = 10 % <br />:roups trimmed and therefore not used <br />in estimating <br />LC50 <br />are marked with an asterisk above. <br />LC50 = 23.82512 <br />Estimated 95 Percent <br />Confidence Limits <br />Lower: 19.06122 <br />Upper: 29.77965 <br />Variance estimate = <br />1.244172E-02 <br />