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f 11119/1999 01.09 2094627935 PRECISION ENVIRO TEC PAGE 03 <br /> I_ INTRODUCTION <br /> The California.Department of SubsW nces Control has adopted regulations(R-45-7 9)which <br /> define criteria for the identification of hazardous wastes. These criteria are codified in Chapter <br /> 11,Article 3 of Title 22 of the California Code of Regulations_ Toxicity to aquatic life, <br /> specifically fish,is one of the criteria used to gauge the hazardous potential of a waste. An acute <br /> Whour bioassay is used to dctmmine the LC50 as deftnad in Section 66261.24(a)(6) of these <br /> regulations. This 96-hour LC50 value serves as the numerical indicator of the toxicity of a waste <br /> to aquatic life. The sample is deemed hazardous if the LC50 is less than 500 mg/L. <br /> This report describes the procedures used and the results obtained for the hazardous waste <br /> aquatic toxicity screening test(s)performed by Block Envirownental Serviw: (BES)for <br /> Precision Enviro-Tech(Project Mame: Sludge'Sarnple). <br /> BPS is an Environmental Laboratory Accreditation Program certified laboratory (#1812). <br /> Z. MATERIALS AND METHODS <br /> 2.1 TEST ORGANISMS <br /> * Fathead Minnow,Pimephales promelas,obtained from a commercial supplier. <br /> 2.2 TEST PROCEDURES <br /> A detailed procedure for this test is outlined in,laboratory standard operating procedures(S OPs) <br /> kept at the BES laboratory. These SOPS are based upon the following references: <br /> a California's Title 22 Code, Section#66261.24(a)(5); Static Acute Bioassay <br /> ,Proceduresfor,Hazardous Waste Samples,Polisini and Miller, 1988, California <br /> Department of Fish and Game <br /> o Guidelines for Performing Static Acute Taxfcity Fish Bioassays in Municfpcal and <br /> Industrial Wastewaters,Kopperdahl, 1976, Califomia Department of Fish and Game <br /> * Standard Methvdsfor the Examination of Water and Wastewater, ISP Edition, <br /> American Public Health,Association, 1995. <br /> 2.3 DATA ANALYSIS <br /> All toxicity testing results will be analyzed using an appropriate statistical method to determine a <br /> LC50 and the corresponding 95% confidence limits. <br /> BES 4 17420 Page 2 of 3 <br />