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Accredited by U S Dept ofCommerce ASBESTOS TEM LABORATORIES, INC CA DOHS ELAP Apr/09/2002 V Miller McCampbell Analytical 110-2nd Ave,#D7 Pacheco,CA 94553 RE LABORATORY JOB # 299-159 Transmission electron microscopy analytical results for I water sample(s) Job Site .lob No 0204071 Enclosed please find results for the TEM analysis of one or more water samples The analytical procedures were performed according to a Modified EPA Method 60014-83 which,while similar in analytical technique,does not meet or fulfill the rigorous requirements of the EPA Drinking Water Standard for various reasons(i a hold time exceeded 48 hours,unrefrigerated shipping, analytical sensitivity>0 2 MFL due to particulate overloading,etc) Prior to analysts,samples are checked for damage,disruption of any chain-of-custody seals,and completeness of accompanying paperwork If no problems are found,samples are then logged-in,each given a unique laboratory number,and a hard copy containing all pertinent information is generated This,and all other relevant paper work are kept with each sample throughout the analytical procedures to assure proper analysis Preparation of water samples is performed within a HEPA filtered,Class 100 air,laminar flow clean bench environment Prior to filtration,water sample Writainers are ultrasonicated,and if necessary,treated with UV light while and ozone gas for three hours to kill and oxidize all organisms and organic materials contained in the water An aliquot of the water sample is pipetted into a special filtration apparatus where contained particulate is collected onto a 0 1 micron pore size polycarbonate(PC)or mixed cellulose ester (MCE)filter The filters are removed from the apparatus and dried A portion of each sample filter is sectioned,placed onto a glass microscope slide, and carbon coated The Filters are further sectioned and placed carbon side up onto 200-mesh copper TEM sample grids in a chloroform or dimethyl sulfoxidelacetone wick washer Sample gnds are placed into a condensation washer,or left in the wick washer,until all filter material is dissolved The TEM grids are removed and placed into labeled grid storage boxes TEM analysis is performed on a Philips EM-300 transmission electron microscope operating at 100 kV Initially,the grid is scanned at low and medium magnifications to insure proper sample loading,and coherence of the carbon support film Then TEM grid openings are analyzed at a magnification of 10,000X All fibers>10 um in length and exhibiting an aspect ratio>3 1 are analyzed Scanning continues until either 100 asbestiform fibers>10um in length are counted, 8 grid openings are analyzed, or an analytical sensitivity of 0 2 mullion fibers per titer(MFL)is achieved Analyzed fibers are subjected to detailed morphological and selected area diffraction(SAED)analysis Fibers indicated as asbestos,or potentially asbestos, are further analyzed by energy dispersive X-ray(EDX)analysis as needed The number of asbestos fibers detected,and other analytical parameters,are then used to calculate the concentration of asbestos in MFL The results are entered into a standard report format and reviewed by the analyst and the laboratory manager before release to the client PTOSTEM ge BORATORIES INC elate only to the samples tested and must not bereproduced,except in full,with the approval of the laboratory ot be used to claim product endorsement by NVL.AP or any other agency of the U S Government --- 1409 FIFTH STREET,SUITE C • BERKELEY,CA 94710 • (510)528-0108 • FAX(510)528-0109 With Offices in Reno, NV(775) 359-3377 and Las Vegas, NV(702)270-2463