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a 19 June 2000 AGE-NC Project No 96-0232 Page 30 of 35 Based on the concentrations of MTBE encountered at the site,the cost for on-site treatment utilizing activated carbon and/or air stripping technologies would be between $0 02 and $0 04 per gallon when discharging into a municipal storm drain/sewer systems Installation of an on-site activated carbon and/or air stripping ground water treatment system typically would cost between$20,000 to $40,000 Based upon the treatment of approximately 9,400,679 gallons, the costs utilizing onsite treatment should range between $208,014 and $416,027 Based on the concentrations of MTBE encountered at the site, the cost for transportation to an off- site waste water treatment facility could be as great as 30 cents per gallon Based upon the installation and treatment of approximately 9,400,679 gallons (see Section 7) of contaminated ground water, the costs utilizing"Pump and Haul' treatment could exceed $2,820,204 9 3 IN-SITU BIOREMEDIATION/BIOENHANCEMENT Bioremediation involves the introduction of hydrocarbon-degrading microbes or supplemental nutrients to enhance naturally-occurring bacteria,through wells into the ground water The microbes . degrade hydrocarbons and spread laterally (and vertically) by migration and reproduction Hydrocarbon-degrading microbes are able to metabolize hydrocarbons and assimilate the resulting organic chemicals into biomass for reproduction and growth Typically,the light-end hydrocarbons are degraded first, followed by heavier compounds 9 3 1 Nutrient Requirements And Toxicity The microbes require certain essential nutrients that are usually available in ground water If supplemental nutrients are required, such as nitrogen and phosphorus as ammonium phosphate in liquid phase,the quantity 1s usually very low However,a Waste Discharge Permit is required by the RWQCB for the infection of nutrients Into ground water, furthermore, the RWQCB has been very reluctant to issue Waste Discharge Permits for nutrient addition Typically,the microbes utilized in bioremediation of ground water are aerobic and commonly require additional oxygen to supplement dissolved oxygen concentrations in ground water Generally, several air sparging or in-well aeration sources are sufficient to provide the necessary oxygen However, in some cases the addition of hydrogen peroxide or other oxygen source Is necessary Advanced CeoEnvironmental,Inc