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i <br /> microbial activity was such that we felt that, with the addition of the horse manure <br /> compost, a sufficient rate of detoxification could be achieved and eventual breakdown of <br /> diesel material would be obtained <br /> For some time, there has been knowledge that many common soil bacteria possess the <br /> ability to metabolically shift to take advantage of fuel hydrocarbons as food when <br /> encountered in soil Frequently, the bacteria involved include many different species of r <br /> pseudomonas Some more recent work has indicated the probability that many types of <br /> fungi are extremely active in the bio-degradation process and may actually present <br /> dominant roles in mineralizing fuel hydrocarbons There was no attempt made in this <br /> investigation to determine what the particular strains of microorganisms were. Although <br /> many organisms have been identified as active in the degradation of petroleum <br /> hydrocarbons, most of them are known as opportunistic utilizers. <br /> The opportunistic utilizer is sunply an organism which is able to use more than one type <br /> of food and has the aWity to shift its diet to whatever food source is most prevalent, 1'e, <br /> a carbon source. In this case, the organisms of interest were those adapted to utilizing <br /> petroleum hydrocarbons as a primary food (carbon) source. In some situations, an , <br /> inoculum may prove to be beneficial; however, the addition of a foreign source of 4u <br /> organisms may:irilnivit the rate of degradation by promoting competition for resources witht <br /> the native or indigenous population." Thein could also be a public relations problems <br /> associated with using an additional inoculums or microorganism in the soil, as there may <br /> be A fear that this added microorganism might be uncontrollable. In most cases, the <br /> native population and character of microorganisms is sufficient using the bioremediation <br /> method under discussion. In the case under discussion, no exotic inoculum or preparation <br /> was involved. The native soil was found to contain a sufficient population of the desired <br /> microorganisms. <br /> Thus bioremediation process operates by accelerating a naturally occurring process which <br /> is always taking place in the soil, however, if the natural process were allowed to continue <br /> unaided, many years would be required to break down the diesel. Alteration of the soil <br /> environment is necessary to provide the conditions necessary for the maximum <br /> mineralization of the contaminants. We found that the soil to be treated was literally <br /> loaded with microorganisms. The crux of successful bioremediation is to promote the <br /> welfare of the mixed inhabitants of the soil, the microorganisms present m the ecosystem <br /> as a whole. Providing an ideal growth environment produces the rapid-rate bio- <br /> degradation necessary to economically remediate diesel-contaminated soil <br /> This particular operation utilized the organisms in the soil which exist in a natural state <br /> of equilibrium. This equilibrium hinges upon the presence of different food sources, the <br /> availability of nutrients, the presence of oxygen and moisture, and other factors Certain <br /> of these factors are always in short supply, thereby,keeping a check on the populations <br /> of the microorganisms. We enhanced the utilization of the organisms by aligning the <br /> eating habits of the microorganism populations to include fuel hydrocarbons and by using <br /> the horse manure compost additive as a supporting nutrient In so doing, limiting factors <br /> • <br />