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tl <br /> „ Issued: 8/23/34 <br /> CENTRAL RESEARCH AND DEVELOPMENT DEPARTMENT <br /> HASKELL LABORATORY FOR TOXICOLOGY APPENDIX Kd <br /> AND INDUSTRIAL MEDICINE <br /> Environmental Biology Section <br /> Microbiology Procedure SOP-PO5 <br /> CULTURE MEDIA PREPARATION <br /> Purpose: <br /> This SOP describes the. general procedure used for making <br /> media and documenting each preparation. <br /> other Relevant 90PIS: <br /> Aquatic Equipment SOP-E13 Weighing with an Electronic <br /> Analytical Balance <br /> Aquatic Equipment SOP-EO8 PH Meter <br /> Microbiology Equipment SOP-Ell Autoclave <br /> i <br /> Responsibility: <br /> It is the responsibility of the person making media to fallow <br /> this SOP. <br /> Equipment: <br /> o Autoclave (Microbiology SOP-Ell) <br /> 15 Electronic Analytical Balance (Aquatic SOP-E13) <br /> a pH Meter (Aquatic SOP-E08) ; <br /> f' 0 Hotplate stirrers <br /> } o Milli-qe Water System <br /> SafetyEq <br /> ui gents <br /> o Blue laboratory coat <br /> o Safety glasses <br /> Safety Considerations: <br /> o Unique to the preparation and composition of each medium. <br /> o See SOP's for each type of equipment listed. <br /> , <br /> Procedure: <br /> - - I - - =- <br /> �' o the - <br /> attached-media preparatiioa-stieet__should-be-filled-out <br /> -- <br /> for each batch of media and only by the preparer. <br /> 13 a The media preparation sheet is placed in the appropriate <br /> laboratory notebook (i.e. next to the study for which the <br /> ii medium is being prepared) . <br /> '9 <br /> �r <br />