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12i1DEC. 10. 1997 i10: 50AML®426(510 5681 CLAYTON LAB N0, 4357 Pd. 187 <br />2. MATERULLS AND METHODS <br />'I <br />2.1 ORGANO BMS <br />H ys using: <br />• Fathead MOM O%S, Pi nephales promelas, obtained from Sticklebacks <br />s Unlimited Vallejo, CA). <br />i - <br />244S PROCEDtnM <br />A led PrOccdurt these tests is outlined in laboratory standard operating <br />Pr"Wum (SOPS) kept at the AES laboratory. These SOPS are based upon the following <br />• if °a' Tat1® 22 Code, Studun 6261.24(3)(6); "Static Acute Sioassay <br />Procedurca for Hatardaus Waste Samples", Polisini and Miller, 1999, <br />California Ike <br />� parent of Fish and Game <br />• "Wideliraes� 'Or Performing Stat Acute Toxicity Fish Biommays in Municipal <br />and IndWfFI61 Wasps Waters", Kopperdahl, F.k.,1976, California Department <br />of Fish and Dame <br />• "Standard 11 ethods far the Examination of Water and Wastewater", 1$th <br />Edition, can Public Health Association, '1992. <br />23 .1iATA ANALYSIJ <br />All tcbcicity testing res us w'jl be ,aeiyzeq usivg the Trimfned-Spearman Karber <br />statiaioal MethoKL This'progmm deteimin®s d LC$O and the corresponcUng 95% <br />' amfi aft limits. <br />