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29,February <br /> AGE-NC <br /> I997 <br /> NC Project No 95-0144 - <br /> Page 10 of 20 <br /> ground water has been im <br /> for inoculation l}acted, strategically located ground water monitor <br /> in <br /> Once in the contaminant I <br /> 'hydrocarbons and spread lateral) Plume,, the bacteria metabolize and therefore g wells can be used <br /> advancing remediation front Y and vertically by migrating degrade <br /> g and reproducing, creating an <br /> Hydrocarbon-degradin <br /> chemicals into blomassgobret rod Tla metabolize hydrocarbons and asst <br /> malate the resulting products The Iight-end hydrocarbons action and growth,and release water and carbon diaxid gasrganic <br /> - hydrocarbon molecules �drocarbons are typical) waste <br /> y consumed f <br /> � first, ollowed b' il Y "heavier" <br /> , <br /> ' 532 <br /> NUTRIENT REQUIREMENTS AND TOXICITY <br /> The bacteria require certain essential nutrients, u' <br /> supP]emcntal nutrients are 5, which are <br /> needed the usually available in native soil If <br /> coni arable to the , quantities and concentrations,are enerafl 'Very p quantities and concentrations utilized for li ill a <br /> g Y ry low, <br /> There is considerable variation in the g griculturaI use <br /> bioremediation types of bacteria used by different remediation cons <br /> The bacteria are cultured and selected for their obeli <br /> ' hydrocarbons They are rri�croaero ultants for <br /> IS not necessa philic{and facultative) � to degrade petroleum <br /> liquid ox Y for the process to occur Exotic forms of Icr , and therefore abund <br /> Ygen)are usually not required Free ox oxygen (such ash ant oxygen <br /> bacteria A small blower del1zrering atmosphericya en increases the metabolic rates ofreactionperoxide or <br /> well can be used to enhaiiee air flan,and ox it to the subsurface at a rate of two to action in the <br /> in unwanted dehydration of the soil, but dehydration en concentrations during the p This hayfive r I per <br /> distilled water to each well ydration can be process y result <br /> ameliorated by monthly injection of <br /> The bacteria are also tolerant to a wide range of soil ca <br /> to 9 5'and electrical conductive <br /> t ty has fettle or no effect unless sodiumris For coninstance, <br /> a weight basis pH can range from 4 <br /> concentrations exceed 5% on <br /> r , <br /> S 3 3 INOCULATION WELLS <br /> The effective radius of influence of inoculation wells r <br /> can be used for inoculationis t <br /> of ground water and the smear zone lY 5 feet Existing <br /> usually obtained for ground water inoculation, since round monitoring wells <br /> migration However A greater radius of influence is <br /> , additional vadose zone Ino elation Water movement c r <br /> inoculation points could be utilized �assist the bacterial ` <br /> points would be needed Temporary <br /> I <br />