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1 IW.- (C) 1989 BIO NATIONAL CORPORATION High populations of introduced microbes follow laws of microbiology that may also apply to augmented feeding of indigenous organisms. It is only necessary to maintain a soil saturation level as low as 10%. In a soil with 30% permeabilityi, this means the gradual introduction of only 6 to 36 gallons of water per cubic yard, with a mean of about 12 gallons per cubic yard of soil. Since this minimal amount of moisture, added gradually, is under negetive pressure, being drawn toward the extraction well, while mechanically recovering excess bottom fluids, there is little chance of the plume being driven downward to the water table. A NOTABLE PARADOX - is the ever expanding data being developed nationwide with regard to bioremediation of gasoline contaminated groundwater. This technology is proving to be the only way to reach the contamination at its micro and macro sox.-ces to eliminate the problem at its point of origin. The alternative method of slow extraction of groundwater for air stripping or carbon filtration is not only uneconomic, uut, by comparison, totally ineffective. However, while microbes can effectively treat groundwater, Much may be accomplished with a combined application of biological soil treatment as described in this application, and efficient water treatment methodologies, such as ozonation utilizing technology introduced here for air scrubbing. MUTATIONS AND OTHgR UNFOUNDh;D PEARS - In addition, certain groups have advanced rather ill-founded obj9ctions to the introduction of so-called alien organisms into remediation sites. These false arguments involve a potential formation of mutant combinations between the natural organisms and those introduced cultures. r; This is contrary to sound biology and belongs in the realm of science •� fiction. Fusion ofgenetic material can take place only under rigidly controlled laboratory conditions using minimal media and cells devoid of walls, the so-called protoplasts. These conditions are impossible in a soil 1 remediation site. In addition, only closely related organisms will exchange genetic material. Further, since these new organisms are highly specialized and attack hydrocarbons for their food; when the food source is exhausted, these organisms will be overcome by the natural soil flora. This is a natural control on the potential clanger of alien organisms taking over. We will be happy to answer any further questions on the subject, and challenge any competent scientist to present contradictory data. THE U. S. ENVIRONlIs7VT.AL PROTECTION AGENCY also states "...it is not necessary under the June 26, 1986 statement of EPA biotechnology policy to report the environmental release of micro-organisms which are naturally occurring, non-engineered and non-pathogenic." 7 HOLT805