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Bioremediation Report 3 - 3 October 1994 <br /> 16 S. Cherokee Lane, Lodi <br /> Comment - Several vapor samples showed a decrease in oxygen and increase in carbon dioxide. <br /> These conditions may indicate bacterial activity, however, no information on these parameters was <br /> collected prior to inoculation. In addition, vapor samples from both inoculated and uninoculated <br /> wells showed the same decrease in oxygen and increase in carbon dioxide. Therefore, it is not <br /> possible to tell if these conditions are a result of inoculation. <br /> B. GeoAudit's Bioremediation Proposal <br /> Despite GeoAudit's "reservations" about the value of the limited inoculation (Pilot <br /> Study), they concluded that the decrease in soil contamination in PI, knowledge of the <br /> bacteria which they have not shared with us, the decrease in oxygen, and increase in <br /> carbon dioxide, all indicate that bioremediation occurred during the Pilot Study. <br /> Therefore, they plan to proceed with the full scale inoculation of soil and ground water <br /> as described in the FRP and addendum, with modifications. <br /> Soil - In the FRP, GeoAudit proposed to inoculate MW1, MW3, MW4, MWS, and VW1 <br /> through VW7 with two well volumes in each well. A total of 432 gallons in the <br /> monitoring wells and a total of 648 gallons in the vapor wells. However, because they <br /> have already inoculated MW4, VWl, VW4, VWS, and VW6 during the Pilot Study, they <br /> have modified the plan. In the new plan, approximately 100 gallons per well will be <br /> • inoculated into MWl, MW3, MW4, and MW5 to a depth of 30 feet, using a packer. <br /> Approximately 100 gallons per well will be inoculated into VW3 and VW7 to a depth of <br /> 35 feet without using a packer. <br /> Comment - In summary, VW2 will not be inoculated at all; VW1, VW4, VWS, and VW6 will not <br /> be reinoculated; MW4 will be reinoculated; MWl, MW3, MW5, VW3, and VW7 will be inoculated <br /> for the first time. GeoAudit did not explain why VW2 will not be inoculated at all as proposed in <br /> the FRP. They also did not explain why MW4 will be reinoculated but not VWl, VW4, VWS, and <br /> VW6. <br /> Comment - The FRP appeared to have the on-site soil contamination near the source fairly well <br /> covered by injection points. They did not address the off-site soil contamination. The FRP and <br /> modified proposal assume the bacteria will migrate between the injection points. <br /> Ground water - The original proposal in the FRP was to inoculate MW7, MW8, MW9, <br /> MW14, and EW1 with 100 gallons each. However, based on new ground water data, <br /> they now propose to inoculate MW8, MW9, MWll, MW14, and EWl. Depending on <br /> data after the initial inoculation, MW12 and MW13 may also be inoculated. <br /> Comment - They have not explained why the upgradient MW6 and the cross gradient MW10 <br /> will not be inoculated, when they both have had contamination. <br /> • Comment - GeoAudit did not explain why new data induced.them to inoculate MWll rather <br /> than MW7 as originally proposed. Both the FRP and the new proposal assume the bacteria will <br /> migrate between injection points upgradient, cross gradient, and downgradient. <br />