Laserfiche WebLink
+t , <br /> ATTACHMENT A <br /> In a letter to Mr. Mark Geweke, dated 1 March 1994, Ms. Elizabeth Thayer of the Central <br /> Valley Regional Water Quality Control Board (CVRWQCB) requested information <br /> concerning the methodolgy for bacteriological analyses. The letter was forwarded to our <br /> Microbiologist, Mr. Jim Hoeltgen, for comments and explanations. The following is a <br /> summary of the process and methodolgy used to identify the bacteria. <br /> ANALYSIS FOR IDENTIFYING NATIVE AND INOCULATED BACTERA <br /> The emphasis for identification is to precisely identify all those bacteria that are pathogens <br /> or secondary pathogens. Because pathogenic bacteria are of economic and health <br /> significance, test suites are available for their rapid determination. The bacteria responsible <br /> for our hydrocarbon remediation are non pathogenic and of little economic importance <br /> beyond hydrocarbon remediation. As a result, suites of tests do . not exist for their <br /> identification, making precise identification more difficult and therefore, increased reliance <br /> is made on colony and cellular morphology. _ <br /> First: Water: Proper dilution prepared for each plate and sample. <br />�. Soil: Samples are extracted with 0.85% sterile saline and the proper <br /> dilution prepared for each plate and sample. <br /> The following are inoculated for soil and water: <br /> TSA PIate <br /> MacConkey (gm-) Agar Plate <br /> MENDO Agar Plate (gm- and Total Coliform, Standard Method 909A) <br /> Bacteria colonies positive for MacConkey and MENDO agar are tested fro Oxidase. Oxidase <br /> negative gram negative colonies are inoculated into the Enteric slate for identification. The <br /> Enteric Slate for the identification of the gram negative Enteric Bacteria: <br /> Glucose fermentation <br /> Gas production from glucose <br /> Presence of Lysine decarboxylase <br /> Presence of Ornithine decarboxylase <br /> HZS Production <br /> Indole formation <br /> Adonitol fermentation <br /> Lactose fermentation <br /> Arabinose fermentation <br /> Sorbitol fermentation <br /> aeobi—1 Audis Service.,I— <br /> GEWEKE mostly—PiWA.0.94 <br />