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Anlab Sparger Hazardous Waste Test: Samples AC 19258, AC19259, AC19260 August 1993 Page ♦ t INTRODUCTION An acute toxicity test was performed on samples AC19258, AC19259, and AC19260. The samples were submitted for testing on ,august 16, 1993. Testing was initiated on August 19, 1993. The test was a 96 hour static acute bioassay for hazardous waste samples using the minnow, Pimephales promelas. The test was performed following procedures outlined in Polisini and Miller (1988). The static nonrenewal test was conducted for a 96 hour exposure period. Each sample was tested using three concentrations to determine whether it should be classified as a hazardous waste according to the criteria established in the California Code of Regulations Title 26, Section 22-66261.24(6). These criteria state that wastes are to be classified as hazardous if an aquatic toxicity test conducted with fathead minnows (Pimephales promelas) results in LC50 values less than 500 PPrn (m9/1)• METHODS Test organisms were cultured at Anlab and were held at 20° C before testing. Fish used for the sample AC19258, AC19259, and AC19260 tests were 34 days old at the start of the tests on August 19, 1993. Test conditions are summarized in TablF 1. The test was conducted in a temperature controlled room at 20 ± 2'C, with a photoperiod of 16:8 L:D. Each test treatment used two replicates with ten organisms per replicate. Test chambers were 5 gallon tanks with 10 liters of test solution per replicate. Concentrations tested were 750 mg/l, 500 mg/I, and 250 mg/I, as recommended for screening assays. Aliquots of the sample were weighed into screw-top Erlenmeyer flasks, 200 ml of dilution water were added to each flask, and the flasks were shaken on a wrist action shaker for six hours. Dilution water was EPA soft water prepared with reagent grade chemicals and Super-O water following EPA protocol (1990). Th a samples were added to the test tanks and the test was initiated by randomly adding ten minnows to each tank. Survival and water quality parameters were measured and recorded daily. Ttls rrtport Is aC0iic8ble only 10 the 3arnoic recervea by the laborator• 're ability of the laboratory is 6mrted to 4pi amoYM paid tOr lis r000rt.Thy report is for:9e _........... . .,.. wt nT.t r_sdoressea ano aeon the^_c-c:inn!I-It!pie caent assumes all liability for tart further distncut on of the report or.is confontr.