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PRINCIPLES OF USE <br /> The basic concepts are not difficult. Systems are seeded with formulations <br /> of carefully matched organisms that can use the various petroleum products <br /> to increase their biomass as energy sources. Application techniques maximize <br /> the biological activity and enable the various organisms to handle the original <br /> array of compositions present as well as those formed through catabolism. <br /> Nearly every program needs to be looked upon as a case unto itself, however, <br /> some guidelines are provided here for consideration. A Solmar Corp. represent- <br /> ative should be consulted prior to initiating any program. In nearly all <br /> instances treatability studies should be conducted by Solmar Corp. to assure <br /> suitability of any program and to determine the product of choice. <br /> Principles need to be borne in mind. The Advanced Bio Cultures Formulations <br /> are based upon preserved microorganisms which become activated when put in <br /> water. <br /> Like all living organisms the various essential elements must be included <br /> in their diet, such as calcium, carbon, iron, magnesium, nitrogen, phosphorous, <br /> etc. Application techniques must take this into account. <br /> Time requirements for completion of any program are dependent upon many differ- <br /> ent factors including the nature of the compositions present, the complexity <br /> of these compositions present, the temperature, pH, salinity, final disposal <br /> techniques, the amount of aeration and agitation available. <br /> For example, bacteria perform most efficiently at 35°C. Their efficiency <br /> • is roughly halved for every 10°C shift. Pasteurization occurs above 50°C. <br /> Similarly a pH of 7 is best with activity being approximately halved for every <br /> shift of I pH unit. Variable pH's have a very deleterious effect on the bac- <br /> teria. <br /> Aeration is crucial. The initial degradation steps require oxygen. Further- <br /> more the bacteria operate far more efficiently aerobically. Aeration also <br /> provides agitation, which increases the oil/water interface surface area drama- <br /> tically. The bacteria then have a greater opportunity to degrade the oils. <br /> Some compositions are more easily degraded than others. Straight chained <br /> compounds generally breakdown much more quickly than aromatics or cross-linked <br /> structures. It may take longer to break down a large number of different <br /> compositions than if fewer are present. The viscosity and quantity of oil <br /> to be degraded influence the time required for the program. <br /> ANTICIPATED RESULTS <br /> Programs vary considerably. Where emulsification is present, many systems <br /> experience demulsification as the hydrophilic-hydrophobic balance of the emul- <br /> sifying agent is disrupted by biodegradation. This can facilitate phase separ- <br /> ation techniques. <br /> On the other hand where distinct phases exist and little degradation has occur- <br /> red treatment with the Advanced Bio Cultures may produce an emulsion, thereby <br /> accelerating biodegradation. <br /> In the treatment of lagoons or sumps degradation may seem slow initially and <br /> then appear to speed up as the system approaches a state of equilibrium. <br /> Following this, sludge from the bottom frequently is freed up, which then <br /> covers the surface. It is subsequently broken down. <br />