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Oil Recycle (optional) Oil/WaterTreatment API Aqueous Influent Chamber Separator Discharge Nitrogen levels should be at least 20 ppm and phosphorous levels at 5 ppm. Presoak the Advanced Bio Cultures for 4 to 12 hours and add at treatment cham- ber. Divide the daily treatment into 4 or 5 applications if feasible. Treatment chambers should have good mixing and at least 2 hours detention. As much as practical is normally recommended. Recovered oil can be reclaimed, burned, or recycled for more complete degradation. DRY LAND SPILLS For areas having oil depths of one foot or less: Under one acre: innoculate with 11b/1.000 sq. ft. One acre or more: innoculate with 50 lbs/acre Two weeks later reseed with 75% of the initial innoculation. Reapply a 50% doseage (of initial innoculation) one month after the second treatment. Reap- ply this same doseage monthly as required. Tars may require heavier seedings. For areas having oil depths exceeding one foot, innoculation should be made at the rate of 25 to 50 pounds per 1,000 cubic yards depending on the nature of the oil and the system. Use the above reseeding schedule. Contact your local representative for the proper program for your specific site. Nitrogen levels must be at least 20 ppm and phosphorous levels at 10 ppm. The bacteria should be added as a slurry prepared with at least two gallons of water per pound of cultures. This should broadcast over the entire surface area. Regular aeration and mixing are necessary for degradation. MONITORING Regular monitoring is necessary. During warm temperatures, significant bub- bling in aqueous systems indicates good microbial activity. Oil quantity monitoring is unusually impractical, particularly when it can be freed up from the bottom of a pond. Bacteria counts using standard plate counts can be useful. There should be significant numbers (30-300) x 10 . Where activity seems low check the following and correct as appropriate. Moisture - Bacteria live and carry on .their life processes in water. Do not allow systems to dry out. Nitrogen - Must always exceed 5 ppm. Phosphorous - Must always exceed 1 ppm. pH - Maximum activity occurs at a pH of 7. This is approximately halved for each 1 pH unit shift. Temperature - Ideal temperature is 95°F. Activity will decrease above or • below this. From a practical standpoint, most programs should be run at 550 to 105°F. If low plate counts occur, check for the presence of inhibitory substances. If not present, reseeding will assure that all species of bacteria are made available to handle residues and ;�etabolities previously produced.