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TABLE OF CONTENTS <br /> Page <br /> r% <br /> INTRODUCTION ................................................................................................................................. 1 <br /> MATERIALSAND METHODS .............:................................................................................................ 1 <br /> Facilities.................................................................................................................................. 1 <br /> TestContainers ...................................................................................................................... 2 <br /> Determination of Water Quality Parameters ............................................................................ 2 <br /> TOXICITY TEST PREPARATION ........................................................................................................ 3 <br /> Receiving and Acclimating Test Fish ...............................:....................................................... 3 <br /> Dilution Water Preparation................................................I..................................................... 3 <br /> Handling and Storage of Waste Sample................................................................................... 3 <br /> WASTESAMPLE PREPARATION ...................................................................................................... 4 <br /> DryWaste Material ................................................................................................................. 4 <br /> Liquid Waste of Low Viscosity.....................................:............................................................ 4 <br /> TOXICITYTESTING ............................................................................................................................ 4 <br /> DosingTest Aquaria................................................................................................................. 4 <br /> Initial Water Quality Measurements.......................................................................................... 4 <br /> Additionof Test Fish................................................................................................................ 5 <br /> Observations........................................................................................................................... 5 <br /> Alkalinity and Hardness Analysis ............................................................................................. 5 <br /> Determination of Test Fish Lengths and Weights .................................................................... 5 <br /> RESULTS .......................................................................................................................I.................... 5 <br /> Standard DOHS Toxicity Screen Testing................................................................................. 5 <br /> REFERENCES ................................................................................................................................... 6 <br /> APPENDICES <br /> A Daily Water Quality Parameters and Live Organism Enumeration <br /> B Fish Length and Weight Measurements <br /> C Sample Analysis Information <br />