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• The Estimated cost for Option 2 is $ 209 000.00. The pricing is inclusive of 4Quarters <br /> of follow up Groundwater Monitoring. A spreadsheet for the estimate costs is provided as <br /> an Appendix A. <br /> O tion 3 —In-situ BiologLc DeUg&tion of Petroleum Contamuiants <br /> This option is an "in-situ" approach in that it uses petroleum-degrading microbes <br /> (naturally occurring bacteria) to degrade the plume in place This would be an enhanced <br /> natural attenuation approach that would use microbes selected from indigenous bacteria <br /> gathered from sampling done on-site <br /> Wright was able to excavate down to a contaminated zone (see Figure 6 for locations) to <br /> obtain some of the most highly contaminated soil whereupon the sample was sent to a <br /> microbiological laboratory for a small bench and data study The bench scale and data <br /> study show that viable petroleum-degrading bacteria are present and can be mass- <br /> produced (See Appendix B) - <br /> From the contaminated soil gathered on:-site, Wright would produce a mass culture of <br /> water borne indigenous bacteria In addition, a nutrient solution would be specifically <br /> formulated for the bacteria from laboratory grade chemicals to initiate and sustain a rapid <br /> microbial breakdown of the hydrocarbon contaminants The bio-solutions would then be <br /> deposited into the vadose soil, capillary frmge and aquifer This would be accomplished by <br /> drilling, and placing, several small 2" slotted PVC pipes around the perimeter of the <br /> identified contamination zone areas Carbon probes would then be placed inside these <br /> PVC pipes The bio-solutions would be introduced into the piping and a small DC current <br /> (Electro-Vectoring Charge) would be initiated through the carbon rods to direct the <br /> bacteria and nutrients that are contained in aqueous solution and are ultimately driven into <br /> and through the contaminated soil zone by the electrical charge This process is repeated <br /> over defined intervals over a period of 3 to 6 months whereupon samples are taken to <br /> confirm contamination reduction. <br /> As this is an in-situ approach, the contaminant degradation would be dependent upon the <br /> indigenous bacteria and nutrient solution efficiency at degradation, and affecting the areas <br /> of interest. It is initially anticipated that this approach would last from 3 to 6 months to <br /> allow for plume degradation. Groundwater monitoring for indicators of degradation(iron, <br /> nitrate, sulfate, oxidation-reduction, oxygen and methane) would be added to the <br /> groundwater monitoring program to monitor effectiveness Initiation of this clean up <br /> option would commence after obtaining the following approval of permits. <br /> • Approval of this CAP by PHS-EHD at CVRWQCB <br /> • Well Drilling permits from PHS-EHD <br /> • Pre-approval of clean up costs from USTCF <br /> • The estimated Cost For Option #3 is $ 133,000.00. The pricing is inclusive of (4) <br /> Quarters of follow up Groundwater Monitoring including degradation indicators A <br /> spreadsheet of estimated costs is provided as Appendix A <br /> Page 15 <br />