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The Estimated cost for Option 2 is S 209,000.00. The pricing is inclusive of(4) Quarters <br /> Of follow up Groundwater Monitoring. A spreadsheet for the estimate costs is provided as <br /> an Appendix A. <br /> 4tion 3 —In-situ Biologic Degradation of Petroleum Contaminants <br /> ' <br /> This option is an "in-situ" approach in that it uses petroleum-degrading microbes <br />{ (naturally occurring bacteria) to degrade the plume in place. This would be an enhanced <br /> natural attenuation approach that would use microbes selected from indigenous bacteria <br /> gathered from sampling done on-site. <br /> Wright was able to excavate down to a contaminated zone (see Figure 6 for locations) to <br /> obtain some of the most highly contaminated soil whereupon the sample was sent.to a <br /> microbiological laboratory for a small bench and data study. The bench scale and data <br /> study show that viable petroleum-degrading bacteria are present and can be mass- <br /> produced (See Appendix B). <br /> From the contaminated soil gathered on-site, Wright would produce a mass culture of <br /> water borne indigenous bacteria. In addition, a nutrient solution would be specifically <br /> formulated for the bacteria from laboratory grade chemicals to initiate and sustain a rapid <br /> microbial-breakdown of the hydrocarbon contaminants. The bio-solutions would then be <br /> deposited into the vadose soil, capillary fringe and aquifer. This would be accomplished by } <br /> 1 <br /> r-- drilling, and placing, several small 2" slotted PVC pipes around the perimeter of the <br /> identified contamination zone areas. Carbon probes would then be placed inside these <br /> PVC pipes. The bio-solutions would be introduced into the piping and a small DC current <br />` (Electro-Vectoring Charge) would be initiated through the carbon rods to direct the <br /> bacteria and nutrients that are contained in aqueous solution and are ultimately driven into <br /> and through the contaminated soil zone by the electrical charge. This process is repeated <br /> over defined intervals over a period of 3 to 6 months whereupon samples are taken to <br /> confirm contamination reduction. <br /> As this is an in-situ approach, the contaminant degradation would be dependent upon the <br /> indigenous bacteria and nutrient solution efficiency at degradation, and affecting the areas <br /> of interest. It is initially anticipated that this approach would last from 3 to 6 months to <br /> allow for plume degradation. Groundwater monitoring for indicators of degradation(iron, <br /> nitrate, sulfate, oxidation-reduction, oxygen and methane) would be added to the <br /> groundwater monitoring program to monitor effectiveness. Initiation of this clean up <br /> option would commence after obtaining the following approval of permits. <br /> • Approval of this CAP by PHS-EHD at CVRWQCB. <br /> • Well Drilling permits from PHS-EHD. <br /> • Pre-approval of clean up costs from USTCF. <br /> + The estimated Cost For Option #3 is S 133,000.00. The pricing is inclusive of (4) <br /> Quarters of follow up Groundwater Monitoring including degradation indicators. A <br /> spreadsheet of estimated costs is provided as Appendix A. <br /> Page 15 <br />