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This will prevent concentrated organic loading and prolong the lifespan of the disposal system. Total Soluble Solids (TSS) originating from organics can also create and enhance biomat formation between the native soil and effluent interface, thus restricting infiltration. The biomat, also referred to as a clogging mat, is a biological, slimy substance which forms on the soil-effluent interface over time. This mat is composed mostly of aerobic and anaerobic bacteria and bacterial products consisting of polyuronides and polysaccharides. The biomat does have one beneficial property of removing approximately 10%of the nitrogen from the effluent. Additional wastewater constituents may consist of coffee,juices or other similar type liquids to the system. It is imperative that no industrial or commercial chemicals, solvents, excessive cleaners, etc. are introduced into the septic system, as well as other items such as cloth, plastic, etc. The new kitchen sink must not contain a garbage disposal and oils and grease must not be poured down the sink drain. All fixtures (faucets, toilets,urinals) must be of the lowest flows available. A water softener should not be installed on the domestic water supply line since the sodium liberated could deflocculate the clays and collapse the soil structure of the effluent receiving soils. 1�ILS§3.3. Table 6 below summarizes concentrations of septic tank constituents. To determine the nitrogen concentration for use in the nitrate loading calculations, an effluent sample was obtained from the second existing septic tank, before effluent disposal. The primary constituent tested was nitrogen. Total Kjeldahl Nitrogen(TKN) is the summation of ammonia-nitrogen(NH4) and organic nitrogen(proteins, amino acids, enzymes). Since the septic tank is predominately anaerobic, nitrogen cannot exist in its oxidized state as nitrate for any length of time in the septic effluent, and if it does, it is quickly reduced to NO or NOZ. If nitrate existed in the tank effluent, this would also be added to ammonium and organic nitrogen to arrive at Total Nitrogen. In addition, septic tank effluent concentrations from various researchers that have been published in Small and Decentralized Wastewater Management Systems, along with septic tank effluent concentrations for the constituents listed by the U.S.E.P.A. are listed in the Table. 10 Chesney Consulting