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The initial October 13, 2007 test results revealed the presence of Total Coliform bacteria. The new well was subsequently chlorinated by the well driller and two additional samples were taken on October 29 and November 6. Both these tests showed the presence of Total Coliform. The well was chlorinated again under strict published guidelines on December 3 and sampled December 4. This sample was take under Chain of Custody to a different laboratory with test results indicating 'T otal Coliform absen All well sampling events were done under strict sampling protocol including well purging, spraying the sample port with diluted bleach, latex gloves, proper sample containers, and refrigerated transport. Calculation of Alkalinity Requirement for Nitrification The nitrate-nitrogen loading calculations on Page 10 are contingent upon the environmental factors required for nitrification to occur. These conditions include soil pore-space oxygen content, soil temperature,pH, electrical conductivity, organic matter, cation exchange capacity, and alkalinity. Alkalinity in wastewater effluent is derived from the domestic well water supply in addition to the introduction of wastes. Nitrification consumes approximately 7.1 mg of alkalinity for every mg of ammonia-nitrogen (NH4 N) oxidized. Nitrification of the average Total Nitrogen (TN) concentration of 113 mg/L would require: 113 mg/L NH4-N x 7.1 mg CaCO3 = 802 mg/L alkalinity. The alkalinity of the underlying aquifers supplying the domestic water supply is much lower than this concentration based upon previous analyses. Therefore, the low alkalinity concentration may theoretically inhibit nitrification to an indeterminable degree. /Mounding Analysis Since the groundwater was measure t 22.8 feet from he sounding of the new on-site domestic well, adverse mounding potential shoul t and inconsequential. Additionally, since a large percentage of sand is known to occur at the deeper depths, this would also in all likelihood, prevent the formation of the mound phenomenon under the disposal area. The critical issue regarding the groundwater elevation is: 1.) There must be sufficient distance between the soil/effluent interface and the highest anticipated depth to groundwater for sufficient treatment of effluent to occur, which is generally accepted to be five feet for leachlines and 10 feet for sumps or seepage pits, and 2.) The water table cannot encroach upon the septic tanks,which may cause buoyancy. A discussion of separation distance is found in the Conclusions Section of this Report. E. SURFACE WATER INFORMATION Storm water management is on-site containment. There should be no impact to percolating effluent water from the septic system. Since the groundwater directional flow is to the west, percolating rainwater runoff from the on-site structures east of the disposal area into the top aquifer mixing layer may create a dilution effect to the downgradient percolating septic system effluent. Groundwater dilution effects can only be truly discerned with complicated groundwater monitoring and modeling. According to a scientific paper authored by Shaw and Turyk (1994), reduction of nitrate concentrations in groundwater can occur primarily through dispersion, or by percolating rainwater recharge. 6 Chesney Consulting