Laserfiche WebLink
C-v <br /> ytoCulture <br /> E N V I R O N M E N T A L <br /> B 1 Q T E C H N O L O G Y <br /> 40 CycoC ulture Internattonal, Inc 1986 <br /> June 1, 1999 <br /> Client: Upgradient Consultants CytoCulture Code: 9943 <br /> Sequoia Workorder #: 99018 Project Description: Valley Motors <br /> Contact. Vic Cherven Tel (530) 677-3055 Fax: (530) 677-3055 <br /> SAMPLES: lour water samples were received on 5/17/99 rrhe sample was assayed that day and stored at <br /> 4°C for any follow up work <br /> Hydrocarbon-Degrading and Total Heterotrophic Bacteria Enumeration Assays <br /> ANALYSIS REQUEST- Bacterial enumn.ration for aerobic petroleum hydrocarbon-degrade rs (broad range <br /> petroleum hydrocarbons gasoline only) and total heterotrophs <br /> CARBON SOURCE Gasoline hydrocarbons were dissolved into the agar as the sole carbon and energy <br /> sources for the growth of hydrocarbon-degrading aerobic bacteria 3n agar plates <br /> •PROTOCOLS: <br /> Hydrocarbon Degraders Sterile agar plates (100 x 15 mm) were ,)repared with <br /> minlrnal salts medium at pH 6 8 with 1 5% noble agar, without an i other carbon <br /> sources or witrients added Plates were inoculated with 10 ml of simple or a log <br /> dilution of each water sample Triplicate plates were inoculated with sample log <br /> dilutions of 10", 10', 10-2 and 10' The hydrocarbon plates were poured and <br /> counted after 7 days incubation The plate count data are reported as colony <br /> forming units (eft) per milliliter(ml) or gram (g) of sample Eacli bacteria <br /> population value represents a statistical average of the plate count data obtained <br /> with inoculations for two of the four log dilutions tested <br /> Heteroirophs Sterile agar plates (100 x 15 mm) were prepared with minimal <br /> salts medium and 2 35% plate count agar at pH 6 8 without any other carbon <br /> source,) or ruitrients added Plates were inoculated with 1 0 ml of ,vater sample, or <br /> a log dilution of the sample, in triplicate at sample dilutions of 10° 10', 10-1, and <br /> 10-3 `Me heterotroph plates were poured and counted after 4 days The plate count <br /> data are reported as colony forming units (efu) per milliliter(rul) or gram (g) <br /> sample Fach enizrneration value represents a statistical average of two of the <br /> three log dilkrtions inoculated in plates <br /> Vox 510 233 OlU2 Pax 510 233 3777 Email CytoQCytoCulture coin <br /> 249 Teu6bivy A�12Me Poor Rtchmond California 94801 382() USA <br />