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AEROBIC <br /> Hydrocarbon-Degrading and Total Heterotrophic <br /> Bacteria Enumeration Assay Results <br /> Client Sample Hydrocarbon Target Total <br /> Number Sample Date Degraders Hydrocarbons Heterotrophs <br /> (cfu/ml) Tested (cfu/ml) <br /> MW-1 09/16/03 2x 102 Gasoline/Diesel 8x 101 <br /> MW-4 09/16/03 2x 101 Gasoline/Diesel 5x 10 <br /> MW-513 09/16/03 3 x 103 Gasoline/Diesel 2 x 104 <br /> MW-8 09/16/03 5 x lO2 Gasoline/Diesel 2x 103 <br /> Sterile Water 09/17/03 0 Gasoline/Diesel 0 <br /> Air Control 09/17/03 0 Gasoline/Diesel 0 <br /> Positive Control 09/17/03 4x 108 Gasoline/Diesel 1 x 109 <br /> Reporting Limit for enumeration data is 1 0 x 101 cfu/ml <br /> A positive control sample of hydrocarbon-degrading bacteria was run concurrently with these <br /> samples using a mixed flask culture of bacteria isolated from contaminated groundwater sites in <br /> • Northern California <br /> Inorganic Chemistry and Nutrient Assays <br /> Analysis Request: Nutrient assays were preformed for pH, dissolved oxygen (DO), oxidation- <br /> reduction potential (ORP), ammonia nitrogen, ortho-phosphate, sulfate, nitrate, alkalinity, and <br /> ferrous iron <br /> Protocol: Spectrophotometric assays were performed to determine the concentrations of <br /> ammonia, ortho-phosphate, sulfate and nitrate The assays follow EPA manual colonmetric <br /> protocols using precalibrated reagents and a Bausch & Lomb spectrophotometer 20 All assays <br /> conform to California CLP and Standard Water & Wastewater Methods <br /> The testing for ferrous iron and alkalinity was subcontracted out to Curtis & Tompkins, Ltd <br /> Analytical Laboratory <br /> 2 <br />