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�1 <br /> the progress of the remediation, BIO-REM utili--4 periodic vapor from the sox month Sam event also indicated a lack of <br /> ,analysis for hydrocarbons from several open )holes through- significant reduction in conll�, inant concentrations. <br /> out the site. Based on these results, SIO-REM submitted a request to the Air `. <br /> The progress and effectiveness of the bioremediation technology Force to re-inoculate the site based on their assessment that <br /> was evaluated by the SITE Program by comparing baseline (time sub-surface lithological conditions inhibited the remedial process. <br /> o)soil contamination levels to contamination levels at one month, In March of 1993 BIO-REM re-inoculated the site by injecting <br /> three months, and six months after inoculation. During each approximately 35,000 gallons of H-10 slurry into 104 boreholes <br /> sampling event, twenty borings, within a highly contaminated deepened to a depth of 23 feet below land surface.The inocula- <br /> portion of the site, were used to evaluate the process. These tion to deeper depths was implemented to overcome the sub- <br /> borings were divided into four depth intervals from four feet BLS surface lithological conditions identified by BIO-REM. In June of <br /> to twenty-five feet BLS. All samples were analyzed for benzene, 1993 a confirmatory sampling event initiated by the Air Force, in <br /> toluene, ethylbenzene, and xylenes (Method SW846 5030/8021) conjunction with the SITE Program, indicated that significant <br /> and Total Recoverable Petroleum Hydrocarbons(TRPH) (Method contamination existed at the site, and that the re-inoculation was <br /> SW846 3550/EPA 418.1). In addition, selected samples were unsuccessful in reducing the target contaminants to the project <br /> analyzed for Total Petroleum Hydrocarbons (TPH), as per the specific dean-up levels.Based on these results,these site activi- <br /> Califomia Leaking Underground Fuel Tanks Manual Method for ties were concluded. <br /> diesel contaminated soils. <br /> Demonstration Results: Results from the Demonstration indi- <br /> A final report will be available in the summer of 1994 <br /> cate that the BIO-REM process was unsuccessful in reducing For Further information: <br /> target contaminants in the soil to the project dean-up levels. <br /> EPA Project Manager. <br /> Baseline sampling indicated that a majority of the soil samples Kim Lisa Kreiton <br /> were significantly higher than the cleanup levels of 130 ppb for U.S. EPA Risk Reduction Engineering Laboratory <br /> benzene and 100 ppm for TRPH. Furthermore, soil samples 26 West Martin Luther King Drive <br /> analyzed one and three months after inoculation did not show Cincinnati, OH 45269 <br /> significant reductions in benzene or TRPH contamination (Table 513-569-7328 <br /> 1). The lack of progress in the remediation prompted concerns <br /> regarding the effectiveness of the technology. It was jointly de- BIO-REM, Ina: <br /> cided between the SITE Program and BIO-REM to collect sixteen David O. Mann <br /> samples(four boreholes) at six months to determine the progress P.O. Box 116 <br /> of the remediation at the predicted end of the project. Results Butler, IN 46721 <br /> 800-428-4626 -U.S.C'MWrCr WK Pfting om= IW3—sBo�ab=116 <br /> Table 1. Frequency Distribution of Benzene and TRPH for Demonstration Samp,rng Events <br /> Benzene (ppb) <br /> Number of Samples <50 50-130 130-1,000 1,000-10,000 10,000-100,000 <br /> Baseline 33 4 13 26 4 <br /> One Month 27 3 20 25 5 <br /> T hree Months 37 3 12 22 6 <br /> Total Recoverable Petroleum Hydrocarbons (ppm) <br /> Vumber of Samples <100 100-1,000 1,000-10,000 <br /> 3aseline 27 33 <br /> 20 <br /> One Month 29 34 17 <br /> three Months 22 30 <br /> 28 <br /> United States BULK RATE <br /> Environmental Protection Agency POSTAGE&FEES PAID <br /> Center for Environmental Research Information EPA <br /> Cincinnati, OH 45268 PERMIT No.G-35 <br /> Official Business <br /> Penalty for Private Use <br /> $300 <br /> EPA/540/MR-93/527 <br />