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OVERVIEW: BIOREMEDIATIOfd OF HYDROCARBONS The biological treatment of hazardous materials has become a major consideration in the field of waste handling. The growing concerns for placement of contaminated soils in off-site disposal areas as well as the increasing restrictions of the landfills has caused owners of the properties to consider on-site remediation. The liabilities have been focused towards the waste generators and the "cradle to grave" regulations leave them liable for off-site waste. The urgency has been placed on industrial and commercial facilities by the U.S. Environcental Protection Agency (EPA) for the nation' s underground storage tank systems. The EPA has developed underground storage tanks (UST) regulations to prevent leaks and spills and to correct the problems caused by them. Also each state is to develop a regulatory programs for USTs that is as strict as the federal regulations. Many businesses are faced with short-term clean-up responsibilities which can be financially devastating. Many states have passed legislation requiring notification of hazardous conditions or users when property is transferred. Deadlines for corrosion protections and spill prevention devices as well as deadlines for leak detection are ®andated by the Environmental Protection Agency. Our proposals to clean-up operations include: • On-site remediation of contaminated soils • No interruption of business • Lower cost than "dig and haul" methods -- usually 50-60% of cost The remediation process includes a biological treatment of hazardous materials. The advantage of enhanced bacterial cultures offer predictable, faster and more cooplete degradation over a wider range of environmental parameters than naturally occurring microbes. Bacterial cultures are developed through the use of selective adaptation and mutation techniques in order to develop strains with enhanced abilities. The natural remediation of the soil is enhanced to overcome the excessive presence of hydrocarbon (oil or gas) due to a leak or spill. Pacific Rio Environmental combines specialized microorganisms with cost- effective, site-specific engineering for the clean-up of hazardous materials contained in gasoline stations, bulk facilities, refineries, underground storage tanks, and soil. The recaedial clean-up of these sites generally involves the use of one or more of our processes. LR 9S RM IMPMMt)I T'S . an in-situ treatment process for waterborne wastes in lagoons and impoundments. The process uses conventional aeration technology with specially developed microorganisms, nutrients, and surfactants along with appropriate analytical monitoring program. With appropriate mixing, suspended sediments can be treated, resulting in a site which can be returning to practical use and is environmentally acceptable. SOILS RM SLUDEIES • surface soil, sand contamination or contamination which is accessible from the surface can be treated with specialized organisms, nutrients and surfactants utilizing soil aeration mixing techniques, and