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When low to moderate concentrations of oil are thoroughly <br /> dispersed within aerobic water, it is unlikely that a lack of <br /> oxygen is a limiting factor. However, in cases where pools <br /> of oil accumulate in soils and sediments, in regions of intense <br /> decomposition and in some groundwater, the development of anoxic <br /> conditions can severely limit the rate of biodegradation. <br /> MINERAL NUTRIENTS <br /> Petroleum is rich in carbon and energy, but deficient in those <br /> mineral nutrients ( nitrogen, phosphorus and iron) necessary <br /> for microbial growth. Consequently, petroleum degrading <br /> microorganisms must obtain their essential mineral nutrients <br /> ' elsewhere . Those projects in which macro- and micro-nutrients <br /> are carefully monitored have shown improved degradation rates. <br /> Nutrients are often supplied in the form of compost or commercial <br /> fertilizer. <br /> ADDITIONAL FACTORS <br /> In addition to the above, two processes are carefully considered <br /> in the biodegradation (microbial metabolism) of petroleum <br /> hydrocarbons . These are sparing and co-oxidation. Both <br /> processes can, and probably most frequently do, occur within <br /> a petroleum spill . For example, acetate, an intermediate product <br /> in hydrocarbon biodegradation, has been found to reduce the <br /> utilization of hexadecane. The basis for this sparing effect <br /> is not well defined, but it certainly occurs for other hydro- <br /> carbons . Such phenomena do not alter the metabolic pathways <br /> of degradation, but determine whether the enzymes necessary <br /> for metabolic attack on a particular hydrocarbon are active, <br /> or even produced. These sparing effects have a marked influence <br /> on the persistence of particular hydrocarbons within a petroleum <br /> mixture and so significantly impact the degradation process . <br /> Co-oxidation is a process in which compounds which otherwise <br /> would not be degraded can be attacked by enzymes due to the <br /> abilities of the individual microorganisms producing those <br /> enzymes to utilize other hydrocarbons within the petroleum <br /> mixture . The complexities of petroleum compounds provide an <br /> excellent chemical environment in which co-oxidation can occur. <br /> Many complex branched and cyclic hydrocarbons are degraded as <br /> a result of the co-oxidation process . It has been found, for <br /> instance, that the degradation of hydrocarbons within a <br /> high-octane gasoline was not in agreement with the degradation <br /> of individual hydrocarbons comprising the mixture. <br /> III . <br />