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J7 <br /> I ' , <br /> ` SEQUOIA ANALYTICAL <br /> 680 Chesapeake Drive • Redwood City, CA 94063 <br /> (415) 364-9600 FAX (415) 364-9233 <br /> :.:. I.......:-..:::::..:.:... .. <br /> Canonie Environmental Client Project ID: 91-106-05/Time Oil-LODI Sampled: 12/18/91 <br /> 7901 Stoneridge Drive, Suite 100 Sample Descript: 055-SP Comp.-5 Received: 12/19/91 <br /> .: Pleasanton, CA 94578 Analysis Method: See below <br /> Attention Dave Collett Lab Number: 1123020Re orted 1/8/92 <br /> STATIC ACUTE HAZARDOUS WASTE BIOASSAY <br /> Statics Species: Pimephales promelas Organisms/Tank: 10 <br /> Cont. Flow Common Name: Fat-head Minnow Replicates: 2 <br /> Mean length: 35 mm Organisms/Conc.: 20 <br /> Mean weight: 0.56 g Tank Depth: 13 cm <br /> Screenings Supplier: Sticklebacks Unlimited Tank Volume: 10 L <br /> Definitive X Acclimation Temp.: 17 degrees C I <br /> Alkalinit , m L Hardness, m L <br /> Initial Final Initial Final <br /> Control 33 32 46 46 <br /> Dilution Water: Synthetic Softwater 100 ppm 33 31 46 43 <br /> 320 ppm 33 37 47 48 <br /> 1000 ppm 32 42 49 53 <br /> i <br /> I Initial 24 Hr 48 Hr 72 Hr 96 Hr <br /> DATE 1-3-91 1-4-91 1-5-91 1-6-91 1-7-91 <br /> FDO C pH DO C pH # M DO C pH # M DO C pH # M DO C pH #M Total <br /> mg/L Temp Units mg/L Temp Units Dead mg/L Temp Units Dead mg/L Temp Units Dead mg/L Temp Units Dead Dead <br /> Control 10.2 17 7,5 8.5 17 7.4 0 8.3 17 7.3 1 8.4 17 7.1 1 8.8 17 7.2 1 1 <br /> 100 pm 9.8 17 7.3 8.1 17 7.3 0 7.9 17 7.1 0 7.9 17 7.0 0 9.0 17 7.2 0 0 <br /> 180ppm .10.1 17 7.5 8.8 17. 7.4 0 8.5 17 7.3 0 8.5 17 7.1 0 9.0 1 17 7.2 0 0 <br /> 320 m 9.9 17 7.3 8.1 17 7.2 0 7.6 17 7.1 0 8.0 17 7.1 0 8.3 17 7.1 0 0 <br /> 560 ppm 10.3 17 7.3 7.6 17 7.2 0 6.4 17 7.0 0 16.5 1 17 6.9 0 8.4 17 7.1 0 0 <br /> 1000 m 10.51 17 1 7.6 8.9 17 7.3 0 B.4 17 7.2 0 8.4 17 7.1 0 8.8 17 7.1 Q 0 <br /> LC-50: >1000 ppm LC-50 Calculation Method: Moving average angle <br /> Remarks: <br /> Analyst: R. Geckler Method Reference: Static Acute Bioassay Procedures for Hazardous Waste Samples, <br /> September 1987, California Department of Fish and Game WPCL <br /> SEQUOIA ANALYTICAL Page 1 of 2 1123020.CAN 10/10/91 <1> <br />� r <br />