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j <br /> �CLEARVIrATER '�nAY_2s 1ss5 <br /> G '.R. '0 �a �' N C. c r' NY'i IVI Irl If1L 1"7�J1LTH � <br /> 'y . . - :. <br /> A <br /> PERMIT/SERVICES <br /> . IN;SITU RESPIRATION TEST°PROTOCOLS <br /> PURPOSE AND.SCOPE x ' <br /> Thsspu'rpose of the in-situ;respiration testis to evaluate` <the feasibility of.biovezitirig ;_- <br /> ' <br /> at and determine oxygen requirements;.for enhanced:biodegxadatiOn in the j <br /> vadose zone_'..In' particular, the ob}ectives.of the test are to, <br /> `Evaluate the natural•soil,.bioactivity to determine'whether, bioremediation'will <br /> be-effective tithe site:—.1f`natural bioactive g is rester in;the contaminated-soil,. . <br /> x . T <br /> { a relative to the uncontaminated soil, then <br /> + ,Determine`the •maximum.rate at which-,naturally,occurring microorganisms:in <br /> the soil can use.oxygen delivered in,air to aerobically, metabolize hydrocarbons. <br /> r x <br /> 4 _ ..ate - -•,:5 ,+ + -. - f . - .; ", - f :,. "i, f ..;. -,-kd -� <br /> Erom the oxygen'..utilizatiOn 'rate, an approximat'e'hydrocarb.on reduction rate .in ' <br /> mg/kg'=day, can be calculated and extrapolated_io' estimate -the.-length of time to ; <br /> complete the cleanup v x xJ k_ r x k <br /> 2. ; <br /> The uttial phase -of the test consists of evalua <br /> uting the initial vadose zone bioactivity., . <br /> r Based on these.results', the soli= is-then flooded;with ambient,air ( Zl% o�cygen). The 1 <br /> . :.subsequent uptake, of the oxygen :aild..pr' tion Of"carbon��dioxide is:monitored , t y <br /> inside tfie''zone of:contam, ii ation arid,compared-to_background biodegradation rates <br /> to evaluate wliefler or not =the".contamination.will-serve,as.an.energy.source for the'L. <br /> indigenous microbial population.: <br /> FIELDPROCEDURES AND EQUIPMENT. . ,3. <br /> Soil Bioactivity Test Procedures Y {_ <br /> �. The soil bioactivity is .detern �r►ed through the evaluation,of the biogenic .gasses <br /> :.F oxygen',(0 and carbon' dioxide (C0z) out,the"site. ;The potential for:in-situ <br /> biodegradation of�:hydrocarbons' in the vadose:zone via bioventing cans Cheri be <br /> � evaluated 'Concentrations of 02 and CO2_along with volatile organic' compound. <br /> f . <br /> concentrations are measured,,.monitoring wells,(with.'screens extended,above the <br /> groundwater fable) and/Or vapor wells at various.locations'inside -and-outside.the . <br /> zone of contamination This'measurement will be collected .prior t& aerating:the } <br /> In Situ Iftespsrahbn Tesf>'Protocols Pagel of b �.. " April 28, 1995 <br /> � h , f e1 r G � i�, <br />