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carbon sources or nutrients added. Triplicate plates were inoculated with 1.0 ml of a 0.2% sodium <br /> pyrophosphate extract of sample at log dilutions 10 3, 10-0, 10 5, 10-6 -710�. The heterotrophic <br /> plates were counted after 3 days of incubation at 30eC. The plate count data are reported as colony <br /> forming units (cfu) per gram (g). Each enumeration value represents a statistical average of the <br /> ' plate count data obtained from two of the four inoculating log dilutions assayed. <br /> ' AEROBIC <br /> Hydrocarbon-Degrading and Total Heterotrophic Bacteria <br /> Enumeration Assa Results <br /> Client Sample Sample Hydrocarbon Target Total <br /> Number Date Degraders Hydrocarbons Heterotrophs <br /> ' cfu/ Tested cfu/ <br /> CT-mw-8- <br /> 80(75.5-75) 6/23/2009 1 x 104 Gasoline/Diesel 6x 106 <br /> Sterile Water 6/25/2009 0 Gasoline/Diesel 0 <br /> Air control 1 6/25/2009 1 0 1 Gasoline/Diesel 0 <br /> ' Positive Control 1 6/25/2009 1 2 x 10' 1 Gasoline/Diesel 3 <br /> Reporting Limit for enumeration data is 1 x 10 cfu/g. <br /> ' A positive control sample of hydrocarbon-degrading bacteria was run concurrently with these <br /> samples using a mixed flask culture of bacteria isolated from contaminated groundwater sites in <br /> Northern California. <br /> 1 <br /> achel utourt QMX „0 <br /> ' Laboratory Tcx hnician Rand von Wedel, Ph.D. <br /> Principal Biochemist <br /> CACytoCu1ture2009\Cyto1ab\Lab reports\PSClndustrielOutsoureingLP\Soil 09-150 <br /> 2 <br />