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1 <br /> Cyto <br /> Culture <br /> ENVIRONMENTAL <br /> 1110 "I ECHNOLOGY <br /> CytoCulture International,Inc. <br /> 249 Tewksbury Avenue <br /> Pt.Richmond,CA 94801 USA <br /> McCampbell Analytical Reporting date: July 29,2009 <br /> Address: 1534 Willow Pass Rd CytoCulture lab login: 09-169 <br /> Pittsburg, CA 94565-1701 Project Number: TMSLLIN1 <br /> Tel: 925 252-9262 Fax: 925 252-9269 <br /> ' E-mail: subdata@mccampbell.com <br /> ' Samples: Five water samples were received on 7/23/2009. The samples were stored at 4°C and <br /> assayed on the same day. Please see the attached chain of custody form. <br /> ' AEROBIC <br /> Total Heterotrophic Bacteria Enumeration Assays <br /> ' Analysis Request: Enumeration of aerobic total heterotrophic bacteria by method 9215A (HPC)/ <br /> Standard Methods 9215B modified. <br /> Carbon Source for Total Heterotrophic Bacteria: Growth medium was prepared with <br /> standard methods total plate count agar(Difco) containing a wide range of carbon sources <br /> ' derived from yeast extract,tryptone, pancreatic digest of casein and glucose. <br /> Protocol for Total Heterotrophic Bacteria: Sterile agar plates (100 x 15 mm)were prepared <br /> with minimal salts and 2.35%heterotrophic plate count agar at pH 6.8 without any other carbon <br /> source or nutrients added. Sets of triplicate plates were inoculated with 1.0 ml of sample (log <br /> dilution 100) and log dilutions of each sample at 10-1, 10 2, and 10-3. The heterotrophic plates <br /> ' were counted after 3 days incubation at 30°C. The plate count data is reported as colony forming <br /> units (cfu)per milliliter(ml) of sample. Each enumeration value represents a statistical average <br /> of two of the four inoculating log dilutions assayed. <br /> 1 <br />