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LABORATORY WORKSHEET <br />Report To: Pe-Me-illjo / K(�r' (coN <br />195 114E245 <br />Lab. No. 2-+ Q S Z 3 <br />Date Received: 0:2 LZZ I I <br />Date Reported: _O :2 /.2 $ / 11 <br />Bioassay Type <br />Static \/ Continuous <br />Screening Definifive <br />Sample Description_ <br />S l uck4 e, •- tj cjj j o f C At ( H S- 0 3 ) <br />Control <br />Test Organism _ <br />Aquaria Volume: 10 liters <br />Fnncum les rromelasO IJ _ Source: <br />Aquaria Depth: r • <br />q Inches <br />Thomas Fish Farm <br />Acclimatization; <br />I Days <br />Ys � °�. � deg. C (F. B — I <br />_ <br />P _ <br />Organism Characteristics - Length (mm): Min: S's, mm Max: <br />No. Fish/Concentration: 10 <br />b <br />Total Chlorine Residual: <br />i"gam le Conductivity.�Cs mhos/cm <br />Dilution water - Source <br />mm <br />Soft Water Hardness Initial: <br />Avg: m Weigh (gm): Min: O . �F qm <br />tax; Q , m C3 m <br />Aeration: Air <br />— m <br />Control Hardness Initial: �} mg/l Final: <br />I Final: m <br />mall Control Alkaline Initial: <br />Alkaline —Initial: <br />3 O ma/I Final <br />4v <br />3 m /) Final: mg/I <br />m /i Control <br />Aeration Rate: 100 bubbles/min Aeration Duration: 96 hrs Tanks: All <br />_ <br />Conductivity 21 S umhos/cm <br />Bioassay <br />Date <br />Control <br />Dilution <br />Conditions <br />Time <br />No. <br />% <br />750 <br />No. % <br />500 <br />250 <br />Dup <br />750 <br />Du <br />500 <br />o:i <br />10 <br />loo <br />10 <br />—1 C>-0 <br />No. <br />lO <br />% <br />too <br />No. <br />to <br />% <br />loo <br />No. <br />10 <br />°!° <br />loo <br />No. <br />10 <br />% <br />100 <br />N <br />1 <br />Uu <br />Organisms <br />CZ S <br />Ick <br />10 <br />100 <br />100 <br />IQ <br />t0 <br />100 <br />(0p <br />0 <br />100 <br />10 <br />100 <br />10 <br />00 <br />l0 <br />10th <br />Surviving <br />oz a6 <br />10 <br />100 <br />0 <br />10 <br />10 <br />100 <br />00 <br />10 <br />100 <br />0 <br />i0 <br />100 <br />10 <br />(00 <br />oz/a-+ <br />10 <br />100 <br />IO <br />100 <br />10 <br />10 <br />100 <br />100 <br />10 <br />10 <br />100 <br />100 <br />10 <br />--15— <br />100 <br />CC): So <br />6.8 <br />IOp <br />Dissolved <br />I S= 30 ;F (6 <br />-4 2 <br />c7 <br />� I <br />Oxygen <br />mg/I <br />I : 2-5�. <br />10' 40 <br />-F.L.3_I <br />b <br />3 <br />� . <br />-T (C, <br />t I : 3e)1.5 <br />�.1 <br />-4.3 <br />-+.I <br />-T.z <br />.2 <br />Start <br />a 20.5°C <br />�2o.s°C <br />.� <br />.b-2o.s°C <br />.20.5 C <br />'F.� 2D. C <br />.6 2o.S°C20. <br />�.3 <br />°C <br />pH <br />--------� <br />24 Hr <br />48 Hr <br />72 Hr <br />IQ_+ C <br />' .3 0. °C <br />. 3 1g .�3 OC <br />'fi.S <br />`f 3 <br />19.E °C <br />2 °C <br />19 OC <br />1.4 Ig. °C <br />. 3 .2°C <br />`( I °C <br />'t.3 <br />`I.2 <br />ly, i- C <br />.?o.a °C <br />°C <br />�. S <br />`4.4 <br />_; <br />19.E °C <br />�°C <br />°C <br />-4. <br />`4.3 <br />-4. <br />(9.'+-°C <br />.2a.z,°C <br />- 3 c—) SPC <br />-4 �- 20. VC <br />Temp <br />96 Hr <br />-4, <br />C <br />. -9 <br />`4 a. <br />I� • <br />. <br />I i�3.9 <br />. 3 <br />I� .a <br />�1 <br />Icf _�J °C <br />. <br />19 . C1 °C <br />- <br />Results - LC50 = <br />% Survival <br />Toxic Units T.U. <br />Observation/Remarks <br />1- Sd mall <br />mg/1 <br />Method of Calculations <br />95% Confidence Limits <br />LC5° Method <br />I/ <br />Laboratory Supervisor <br />