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i SEQUOIA ANALYTICAL 819 Striker Avenue, Suite 8 • Sacramento, CA 95834 (916) 921 9600 • FAX (916) 921-0100 Groundwater Technology Client Project ID Manteca Bean Sampled May 21, 1992 ry 1401 Halyard Drive, Suite 140 Sample Descript Water, RW Received May 22, 1992 West Sacramento, CA 95691 Analysis Method See below Attention Scott Gable Lab Number 205-0560 Reported Jun 4, 1992 STATIC ACUTE EFFLUENT BIOASSAY StaticSpecies Gasterosteus aculeates Organisms/Tank 10 Cont Flow= Common Name 3-S ins Stickleback Replicates 1 Mean length 30 mm Organisms/Conc 10 Mean weight 03 g Tank Depth 13 cm Screening= Supplier Sticklebacks Unlimited Tank Volume 10 L Definitive X Acclimation Temp 20 degrees C Alkalinity, m L Hardness, m L Control 250 410 10% 270 400 Dilution Water Synthetic Hardwater 18% 280 400 32% 310 400 56% 330 390 100% 380 340 Initial 24 Hr 48 Hr 72 Hr 96 Hr DATE 5/22/92 5 23 92 5/24/92 5/25/92 5 26 92 DO C pH DOC pH ll# M DO C pH # M DQ C pH 1# M DO C I pH # M Total mg/L Temp Units mg/L Temp Units Dead mg/L Temp Units Dead mg/L Temp Units Dead mg/L Temp Units Dead Dead Control 89 20 83 82 20 83 0 77 20 183 0 77 20 82 0 75 20 183 0 0 10% 94 20 83 84 20 83 0 76 20 83 0 79 20 83 0 76 20 83 0 D 18% 91 20 83 80 20 82 0 74 20 183 0 78 20 83 0 75 20 83 0 0 32% 88 20 82 81 20 82 0 85 20 83 0 77 20 84 0 74 20 84 0 0 56% 86 20 8217920 82 0 75 20 84 0 J :W20 84 0 77 20 84 0 0 100% 79 20 83 87 20 83 0 63 20 85 0 20 83 0 166 20 84 0 0 LC-50: > 100% LC-50 Calculation Method Moving average angle Remarks Analyst D Geor a Method Reference Methods for Measuring the Acute Toxicity of Effluents to Freshwater and Marine Or, anisms, 3rd Ed , EPA 600%4-85/013 EQUOIA ANALYTICAL 711zVJ' 1", Michael R Giles Laboratory Director 205-0560 GRW <1>