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r <br /> 1.� Nave <br /> oxygen and so the availability of oxygen must be considered <br /> �- as an important limiting factor for petroleum degradation. <br /> When low to moderate concentrations of oil are thoroughly <br /> dispersed within aerobic water, it is unlikely that a lack of <br /> oxygen is a limiting factor. However, in cases where pools <br /> of oil accumulate in soils and sediments, in regions of intense <br /> decomposition and in some groundwater, the development of anoxic <br /> conditions can severely limit the rate of biodegradation. <br /> MINERAL NUTRIENTS <br /> L <br /> Petroleum is rich in carbon and energy, but deficient in those <br /> mineral nutrients (nitrogen, phosphorus and iron) necessary <br /> for microbial growth. Consequently, petroleum degrading <br /> ~ microorganisms must obtain their essential mineral nutrients <br /> elsewhere. City projects have enjoyed superior degradation <br /> rates, in part, as of the utilization of mineral rich compost <br /> as a soil amendment. <br /> ADDITIONAL FACTORS <br /> In addition to the above, two processes are carefully considered <br /> in the biodegradation (microbial metabolism) of petroleum <br /> .r hydrocarbons. These are co--oxidation and sparing. Both <br /> processes can, and probably most frequently do, occur within <br /> a petroleum spill. For example, acetate, an intermediate product <br /> in hydrocarbon biodegradation, has been found to reduce the <br /> utilization of hexadecane. The basis for this sparing effect <br /> is not well defined, but it certainly occurs for other <br /> hydrocarbons. Such phenomena do not alter the metabolic pathways <br /> •r of degradation, but determine whether the enzymes necessary <br /> for metabolic attack on a particular hydrocarbon are active, <br /> or even produced. These sparing effects have a marked influence <br /> on the persistence of particular hydrocarbons within a petroleum <br /> mixture and so significantly impact the degradation process. <br /> Co-oxidation is a process in which compounds which otherwise <br /> would not be degraded can be attacked by enzymes due to the <br /> abilities of the individual microorganisms producing those <br /> enzymes to utilize other hydrocarbons within the petroleum <br /> mixture. The complexities of petroleum compounds provide an <br /> excellent chemical environment in which co-oxidation can occur. <br /> Many complex branched and cyclic hydrocarbons are degraded as <br /> a result of the co-oxidation process. It has been found, for <br /> .r instance, that the degradation of hydrocarbons within a <br /> high-octane gasoline was not in agreement with the degradation <br /> of individual hydrocarbons comprising the mixture. <br /> y <br /> Proprietary findings to date support the hypothesis that <br /> excellent degradation potentials for petroleum contaminated <br /> v <br /> r <br />