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Lab No.: <br />Client/ID <br />,AD MINNOW HAZARDOUS WASTE <br />SCREEN BIOASSAY <br />TEST SUMMARY <br />Species: Pimephales promela�. <br />Fish weight (gm): av: 0.) y ; in: 0. a Y ; max: ,9,1 �. <br />Reference Toxicant: SDS con acted monthly. <br />Test chamber volume: 10 lite <br />Temperature: 20 +/_ 2°C. <br />Aeration: none, unless D.O. drops below 5.0 mg/l. <br />Number of replicates: 2. <br />Dilution water: Soft reconstituted water (4048 mg/l CaCO,). <br />T Aquatic <br />Tasting <br />QC � <br />Source: In -Lab Culture. <br />Regulations: CCR Title 22. <br />Test Protocol: California F&G/DHS 1988. <br />Endpoints: Survival at 96 hrs. <br />Test type: Static. <br />Feeding: None. <br />Number offish per chamber: 10. <br />Photoperiod: 16/8 hrs light/dark. <br />TEST DATA <br />INITIAL 24 Hr 48 Hr 72 Hr %Hr <br />Date/Time: 00-4 /.)o 1 -3l -I! loco 1 2-1-1&, fol S 2-2-t6 10'a 2- -11 <br />Analyst: <br />°C DO pH °C DO pH #D °C <br />DO <br />pH #D* <br />D°C DO pH <br />#D <br />°C DO pH OD <br />Control A <br />�. 6. f.0 S 4 .6 .9 O <br />i' ?o 8.7 <br />0 <br />Control B <br />2> fr. v •. y B %• G <br />a- <br />U , 6 $• i% <br />© <br />a• B• 7 7• <br />400 mg/l A <br />Z• 1 B 3.o L S f. 2 7_ t 0 <br />E • S 4, V 77© <br />9.5 7 <br />400 mgtl B <br />X. 1t�I J.. L / -S 0 <br />$%3 <br />-V I if -f 77 <br />© <br />11L.5 Z <br />750 mg/l A <br />110XY Its'. Vi g, 7 T- 8 7G. <br />$•-t <br />Z a ( C. _ <br />(�L5- <br />t• s 1791 <br />750 m B <br />1 , t )- 3v, 9. / 0 <br />�/ �. S :,S <br />O <br />1771 <br />Comments: <br />Extraction method: Mechanical shaking <br />✓ . <br />None (aqueous solution) LaLI . <br />Dissolved Oxygen (DO) readings in mgfl Oz. <br />Test Aerated: No <br />CONTROL HIGH CONCENTRATION <br />Alkalinity Hardness Alkalinity Hardness <br />InitialKill <br />mgACaCO� y 6 mgACaCO, 3 mgliCaCO, 1 f mgnC.CO,Final maA aco, 'Y7 mSA CaCO, 3 S mo CaCO, y > mgA C.CO, <br />RESULTS <br />the checked ult applies based on fish survival rates of this test- NA - nota licable <br />PASSED LC50 > 750 mg/l (<40% dead in 750 mg/1 conc.) <br />FAILED a 40% dead iu 750 mg/l (close to pawing- definitive test recommended) <br />FAILED LC50 < 400 mg/l (>60% dead in 400 mg(1 conc.) <br />Page 3 of 6 <br />2/3/2016 <br />U P_005985 <br />