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;AD MINNOW HAZARDOUS WASTE <br />SCREEN BIOASSAY "T Aquatic <br />Testing <br />Lab No.: Al L n 1 2 qq/ -ov/ <br />QC LebOratwlea <br />Client/ID: _7A <br />TEST SUMMARY <br />LC50 > 750 mg/l (<40% dead in 750 mg/l conc.) <br />Species: PimephalesproWlas. <br />Source: In -Lab Culture. <br />Fish weight (gm): av:_0,2_�; min: 0.2 Y ; max: & j <br />Regulations: CCR Title 22. <br />Reference Toxicant: SDS conducted monthly. <br />Test Protocol: California F&G/DHS 1988. <br />Test chamber volume: 10 liters. <br />Endpoints: Survival at 96 hrs. <br />Temperature: 20 +/- 2°C. I <br />Test type: Static. <br />Aeration: none, unless D.9. drops below 5.0 mg/l. <br />Feeding: None. <br />Number of replicates: 2. <br />Number offish per chamber: 10. <br />Dilution water: Soft reconstituted water (4048 mg/l CaCO,). <br />Photoperiod: 16/8 hrs light/dark. <br />TEST DATA <br />INITIAL 24 Hr 48 Hr <br />72 Hr 96 Hr <br />DatelTime: / i+'f • o 011 0)0 2-t-1it IG ( <br />?-Z - 16 /O)o 2-3-'16 lol S <br />/ — <br />Analyst: <br />°C DO H °C DO pH p D °C DO pH <br />k D °C DO pH k D •C DO pH A D <br />Control A �, y, lr a %.S & l 7 -S <br />.f $- S 1.7 7. 1 0 <br />Control t [•> 41:0 2.• x• 7R b <br />?o. &l s.7 0 <br />TID <br />400 mg/1 A 1Q•0 2o.S t <br />'f c1 S <br />aoomgIlBt:. PO 4s .0 0 <br />d <br />L.S : a,s / <br />750 mg/l A la P .) = V . t . f 2^ -7 <br />lo. S 8 • %4 S Jh �( 14 0 <br />750m B 2 to t•a s. I -i; I v2atS <br />87 8 l7 y >s S 77 <br />Comments: Extraction �ethod: Mechanical shaking ✓ . <br />None (aqueous solution) LvL9 . <br />Dissolved Oxygen (DO) readings in mg/l 0, Test Aerated: <br />/ No <br />C NTROL HIGH CONCENTRATION Total Number Dead <br />Alkalinity Hardness Alkalinity <br />Hardness Control (7 /20 <br />Initial .3 ) men C.Cb� �y 6 men C.co, men C&CO, <br />y! men e,Cq 400 mg/l /20 <br />Final men c.cp, men Caco, 3 2 men CICO, <br />men cavo, 750 m 0 /20 <br />Page 3 of 6 2/3/2016 <br />U P_006109 <br />the checked <br />RESULTS <br />result a lies based on fish survival rates of this test; NA - not a cable <br />PASSED <br />LC50 > 750 mg/l (<40% dead in 750 mg/l conc.) <br />�t/J+ <br />FAILED <br />Z40% dead in 750 mg/l (close to passing- definitive test recommended) <br />FAILED <br />LC50 < 400 mg/l (>60% dead in 400 mg/l conc.) <br />Page 3 of 6 2/3/2016 <br />U P_006109 <br />