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FATHEAD MINNOW HAZARDOUS WASTE <br />SCREEN BIOASSAY <br />Lab No.: ,4 / L o / t 1 ej —ri• ! <br />Client/ID: r.4 710 - L 91 rC q -F <br />TEST SUMMARY <br />Species: Pinwphales promelo. <br />Fish weight (gm): av: D. ) d ; min: D. 2 Y ; max:_p��. <br />Reference Toxicant: SDS conucted monthly. <br />Test chamber volume: 10 lite - <br />Temperature: 20 +/- 2°C. <br />Aeration: none, unless D.O. dr�ps below 5.0 mg/l. <br />Number of replicates: 2. <br />Dilution water: Soft reconstituted water (4048 mgA CaCO3). <br />TEST DATA <br />RqE <br />Tatodu <br />Source: In -Lab Culture. <br />Regulations: CCR Title 22. <br />Test Protocol: California F&G/DHS 1988. <br />Endpoints: Survival at 96 hrs. <br />Test type: Static. <br />Feeding: None. <br />Number offish per chamber: 10. <br />Photoperiod: 16/8 hrs light/dark. <br />Date/Time: <br />Analyst: <br />INITIAL <br />24 Hr 48 Hr <br />72 Hr <br />% Hr <br />t i0-! Wo !-3/-/ <br />° C DO pH ° C DO pH #D OC DO pH # D *C DO pH # D OC DO pH # D <br />Control A <br />�- S f,a <br />,.r <br />m'� <br />), <br />Page 3 of 6 2/3/2016 <br />Control B <br />2 t fc. <br />g� %. o <br />.`( <br />. 6 >r•' ¢ %. g <br />0 <br />. 6 i <br />, 4 <br />00 mg/1 <br />[750 <br />j. I •! O <br />Zfi ^� <br />7- 6 <br />to. s r. t <br />7) <br />00 mg/t <br />jAb. <br />g, % <br />%� .O Z S 0 <br />- d <br />! 1. <br />O <br />� <br />mg/1 <br />7 <br />b•Y �. l % <br />�j• D <br />( i. .0 <br />2-s >r� `( <br />O <br />750 m B <br />Comments: <br />•1 til 71 y �.� b t• / <br />Extraction method: Mechanical shaking ✓ . <br />None (aqueous solution) LvL9 . <br />Dissolved Oxygen (DO) readings in mg/l O, Test Aerated: / No <br />O <br />% !T <br />CONTROL, HIGH CONCENTRATION <br />Alkalinity Hardness Alkalinity Hardness <br />Initial j 3 mg/l CacO, y 6 men CaCo, )y mgn CaCo, y y mgn CacO, <br />Final 3 Y - mgn CaCO, V 7 men CacO, 3 6 men COCO, �{ mgn CaCO, <br />Total Number Dead <br />Control 0/20 <br />400 mg/1 O /20 <br />750 m 0 /20 <br />the checked ✓ <br />RESULTS <br />ult applies based on fish survival rates of this test• NA - nota licable <br />PASSED { <br />LC50 > 750 mg/l (<40% dead in 750 mg/l cont.) <br />FAILED <br />Z40% dead in 750 mg/1 (close to passing- definitive test recommended) <br />,.r <br />FAMED <br />LC50 < 400 mg/l (>60% dead in 400 mg/1 conc.) <br />Page 3 of 6 2/3/2016 <br />