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FATHEAD MINNOW HAZARDOUS WASTE <br />SCREEN BIOASSAY <br />Lab No.: 4 /Z o3o l <br />Client/ID: _-j <br />TEST SUMMARY <br />Species: Pimephales promellas. <br />Fish weight (gm): av:�g l; min: 0. _ max:,61. . <br />Reference Toxicant: SDS conducted monthly. <br />Test chamber volume: 10 liters. <br />Temperature: 20 +/- 2°C. I <br />Aeration: none, unless D.O. drops below 5.0 mg/l. <br />Number of replicates: 2. <br />Dilution water: Soft reconstituted water (40-48 mg/I CaCO,). <br />TEST DATA <br />---m <br />RECEIVEr <br />FEB 18 2014 <br />TNMENTAL <br />APARTMENT <br />ratories <br />Source: In -Lab Culture. <br />Regulations: CCR Title 22. <br />Test Protocol: California F&G/DHS 1988. <br />Endpoints: Survival at 96 hrs. <br />Test type: Static. <br />Feeding: None. <br />Number of fish per chamber: 10. <br />Photoperiod: 16/8 hrs light/dark. <br />Date/time: <br />Analyst: <br />INITIAL <br />24 Hr <br />48 Hr <br />72 Hr IF <br />96 Hr <br />3 J me caco 17Te CaCO, <br />-3 me CacO, 2- me Caco' <br />Final <br />Zi� <br />x-/3`/2 o <br />°C DO p <br />°C DO pH # D <br />°C <br />DO pH <br />I #D <br />°C <br />1 160 1 pH # D <br />°C DO pH #13 <br />Control A <br />1'l. f{ . 7 Z <br />V f, 7 77 v <br />0z,4% <br />G%. U <br />Control B <br />IIt, c( 8aS � <br />1 .r 7 7 C) <br />tq % <br />400 mg/I A <br />ly, 1 9. ( T <br />j I if,9 -1 <br />-k. <br />1 71 <br />0 <br />S <br />o. g 6 2 7 0 <br />400 mg/1 B <br />11. )t S.1 % <br />Z 1, i V <br />1-, -)1 <br />q. 3 17S <br />0 <br />( <br />8 <br />� ( 171 O <br />750 mg/1 A <br />(Y, 3 4 ( 7• <br />1q � 7.9 1, � 0 <br />4,1 <br />%.7 7 7 <br />0 114,9 <br />17,-2 <br />14, 11 s 7 1175 <br />750 m B <br />11. N :T:0 7. <br />119 ,41 `� '1, t1 <br />1'1.9 <br />R y 7.7 <br />�� .7 <br />(f. 6 q. 7S <br />Comments: Extraction method: Mechanical shaking X <br />None (aqueous solution) — <br />Dissolved Oxygen (DO) readings in mg/l OZ. Test Aerated: / No <br />Total Number Dead <br />Control 0 /20 <br />400 mg/1 O /20 <br />750 mg/l 0 /20 <br />RESULTS <br />the checked result applies based on fish survival rates <br />CONTROL <br />HIGH CONCENTRATION <br />Alkalinity Hardness <br />Alkalinity Hardness <br />Initial <br />3 J me caco 17Te CaCO, <br />-3 me CacO, 2- me Caco' <br />Final <br />.0 CaCo, me CeCO, <br />7 me Caco, .5,) me Caco <br />Total Number Dead <br />Control 0 /20 <br />400 mg/1 O /20 <br />750 mg/l 0 /20 <br />RESULTS <br />the checked result applies based on fish survival rates <br />PASSED LC50 > 750 mg/I (<40% dead in 750 mg/l conc.) <br />I A <br />FAILED I z40% dead in 750 mg/1 (close to passing - definitive test recommended) <br />NA <br />FAILED LC50 < 400 mg/I (>60% dead in 400 mg/I conc.) <br />Page 17 of 20 <br />11/26/2013 <br />