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'Mar 22 05 07: 36a KedW, <br /> ardney 209945 p. 3 <br /> - "%itKJET FAX <br /> P- 2 <br /> L INTRODUCTION <br /> The California Depaturient of Substances Control has adopted regulations (R-45-7g)whish <br /> define criteria for lite identification,of hazardous wastes, These criteria arc codified in Chapter <br /> Article 3 of Title 22 of the California Code of Regulations. Toxicity to aquatic <br /> speci ica7ly fish, is one of'the criteria used to gauge the ibazardous potential of a waste. An acute <br /> 96-.Motu bioassay is used to detez7minc the LC50 as defined in Section 66261.24(a)(6)of these <br /> regulations, This 96-hour LC50 value serves as the numerical itt.dicator of the toxicity of a waste <br /> to aquatic life. "lie sample is deemed hazardous if the 1,C50 is less than 500 nng/L. <br /> This report describes the procedures used and the results obtained for the 1a,-ardous waste <br /> aquatic toxicity screening tcst(s)performed by Block Env.iro=ental Services(DES) for <br /> Precision Enviro-Tech, <br /> BES is an Environmental laboratory Accreditation Program certified laboratory <br /> 2. MATERLkLS AND VMTRODS <br /> 2_1 TEST ORGANISMS <br /> + Rainbow Trout(OncnrhYnchus mykiss),obtained from a eornme=ial supplier. <br /> 2.2 TEST PROCEDT RES <br /> A detailed procedure for this test is outlined in laboratorystandard standard operating procedures(SOPS) i <br /> kept at the BES laboratory. These SON are based upon the following references; <br /> • California's Title 22 Code, Section #66261.24(a)(6): Static Acute.bioassay <br /> Procedures for Hazardous Waste SamPles,Polisini and Miller, 1988, California <br /> Department of Fish and Game <br /> • GuWeline,s for Performing Static A cute Toxicity Fish Biotxssays in Municipal and <br /> Industrial Wastewaters, Kopperda hl, 1976, California De <br /> Standard Methods for the Examination of'Water and Wastewater, g t of Edi Edish and tion, <br /> American.Public Hcalth Amociation, 1995. <br /> 2.3 DATA A1NAj.XSIS <br /> All toxicity testing results wil I.be analp ed using an appropriate statistical;method to determine a <br /> LC50 and the corresponding 95%confidence limits. <br /> BES 422001-02 <br /> Page 2 of 3 <br />