Laserfiche WebLink
J <br /> D. Microbiolo Y <br /> Certification requirements of the California Department of <br /> Health Scrviccc control the principal features of micr„- <br /> biology QC. These features include daily recording of all <br /> incubator temperatures, recording and filing of the <br /> autoclave performance record, sterilization of sample <br /> containers, application of a dechiorinating agent to sample <br /> j containers, and monthly performance of a completed coliform <br /> test to verify routine confirmed coliform results. <br /> An annual water suitability test is run to make sure the <br /> purified water used in media preparation contains no growth <br /> promoting or inhibitory substances. An inhibitory residue <br /> t test is carried out annually on glassware to verify that <br /> I routine cleaning procedures will not adversely affect <br /> results. <br /> ! F. Fish Bioassay <br /> Fish toxicity bioassays are carried out according to <br /> r California Department of Fish and Game Guidelines, kising <br /> I specified test organisms, sample dilutions, and sample <br /> volumes. They are conducted in a constant temperature room <br /> in which fish are. acclimated for seven days prior to use in <br /> I tests. Checks performed before and during tests on the <br /> control tank and on all dilutions <br /> dissolved ox arti pti, temperature, and <br /> oxygen level. Freshwater bioassays are checked <br /> for alkalinity and hardness while saline or brackish <br /> bioassays are checked for conductivity at the beginning of <br /> the test. A test is considered invalid if there is more <br /> than .108 mortality among control fish during the test. <br /> Temperatures of test solutions must be within the specified <br /> test range for the organism used. Dissolved oxygen must <br /> not fall below 708 saturation. Confidence limits are <br /> established using the Lichfield-Wilcoxon method of <br /> evaluating dose-effect experiments. <br /> } <br /> VI-3 <br />