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1 <br />' 10 June 1999 <br /> AGE-NC Project No 97-0290 <br />' Page 22 of 26 <br />' organic chemicals into biomass for reproduction and growth Typically, the light-end hydrocarbons <br /> are degraded first, followed by heavier compounds <br />' 8 3 2 Nutrient Requirements And Toxicity <br /> I The microbes require certain essential nutrients commonly available in ground water If <br /> supplemental nutrients are required, such as nitrogen and phosphorus, both available as ammonium <br /> phosphate in liquid phase, the quantity is usually very low However, a Waste Discharge Permit is <br />' required by the CVRWQCB for the injection of nutrients into ground water <br /> Typically,the microbes utilized in bioremediation are aerobic and usually require additional oxygen <br />' to supplement dissolved oxygen concentrations in ground water Generally, several air sparging or <br /> in-well aeration sources are sufficient to provide the necessary oxygen However, in some cases the <br /> addition of other oxygen sources is necessary <br /> I8 3 3 Monitoring Activities <br /> After inoculation, the inoculation wells and ground water monitoring wells should be monitored for <br /> I several months by periodic field measurements of organic vapor, carbon dioxide and oxygen <br /> Ground water samples should be collected and analyzed for petroleum hydrocarbons, bacterial <br /> populations and nutrient availability if monitoring indicates areas of slow remediation, additional <br /> Iinoculations may be required <br /> I8 3 4 Feasibility Studies <br /> Results of bioremediation pilot tests at other sites in California indicate that lighter petroleum <br /> hydrocarbons,such as gasoline compounds are more readily biodegraded than longer-chain(heavier) <br /> hydrocarbons, such as diesel fuel compounds Feasibility studies have not been performed at the <br /> subject site <br /> I8 3 5 Duration <br /> Cleanup durations for in-situ bioremediation will vary, depending upon specific site characteristics <br /> IBased ofithe relatively high concentrations of dissolved hydrocarbons at the site, a treatment period <br /> • of 24 to 36ns estimated <br /> ? <br /> Advanced GeoEmironrnental,inc <br /> I <br />