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CHLORDANE: METHOD 5510, Issue 2, dated 15 August 1994 - Page 2 of 4 <br /> REAGENTS: EQUIPMENT: <br /> 1. Toluene, distilled in glass. 1. Sampler: 37-mm, 0.8-Nm pore size cellulose <br /> 2. Hexane, distilled in glass. ester membrane filter supported by a stainless <br /> 3. Chlordane, 95%.* steel screen in a two-piece filter cassette <br /> 4. Calibration stock solution, ca. 6 mg/mL. holder plus a 10 cm x 8-mm OD x 6-mm ID, <br /> Dissolve 10 mg Chlordane in 1 mL toluene. 20/40 Chromosorb 102 tube (front = 100 mg; <br /> NOTE: Since Chlordane is available only as back = 50 mg), separated by 3-mm silanized <br /> a mixture, standardize the solution as glass wool plug, flame sealed ends with <br /> follows: plastic caps. Pressure drop across the tube at <br /> a. Dilute 10 pL calibration stock solution 1 Umin must not exceed 2.5 mm Hg. Filters <br /> to 10 mL. and tubes are commercially available. <br /> b. Analyze by steps 11-13. 2. Personal sampling pump, 0.5 to 1 Umin, with <br /> c. Divide combined area of Chlordane peaks flexible connecting tubing. <br /> (Fig. 1) by total area of all peaks to 3. Gas chromatograph,electron capture detector, <br /> determine fraction Chlordane, f. integrator and column (page 5510-1). <br /> 5. Internal standard, p,p'-DDT, 98%.* 4. Vials, scintillation, 20-mL, PTFE-lined caps. <br /> 6. 95% Argon/5% methane mixture, purified. 5. Syringe, 10-NL, readable to 0.1 NL. <br /> 6. Flasks, volumetric 10-mL. <br /> 7. Bottles, 60 mL, 40-mm ID, straight-sided with <br /> a PTFE-lined cap for extracting filter holder <br /> and screen. <br /> See SPECIAL PRECAUTIONS. 8. Stopwatch. <br /> 9. Manometer. <br /> 10. Pipets, 1- and 10-mL an other convenient <br /> sizes for preparing standards. <br /> 11. Tweezers. <br /> SAMPLING: SPECIAL PRECAUTIONS: Chlordane and p,p'- <br /> DDT are toxic and rapidly absorbed through the <br /> 1. Calibrate each personal sampling pump skin [4]. Use gloves and eyeglasses to avoid <br /> with a representative sampler in line. direct contact with these compounds. Handle <br /> 2. Assemble the sampler and connect the these chemicals and organic solvents with care in <br /> sorbent tube (ends broken just before the the laboratory hood. Chlordane is a potential <br /> connection) to the sampling pump with human carcinogen [5]. <br /> backup section nearest the sampling pump. Keep <br /> the sampler in a vertical position during sampling. <br /> 3. Sample at an accurately known flow rate between 0.5 and 1 Umin for a total sample size of 10 <br /> to 200 L. <br /> 4. Remove the sorbent tube from the outlet of the cassette and connect it to the inlet side of the <br /> cassette. Cap the open end of the sorbent tube and plug the outlet of the cassette. Ship <br /> sampler with appropriate blanks to laboratory. <br /> 5. In separate package, ship bulk sample of the suspected material. <br /> SAMPLE PREPARATION: <br /> 6. Separate the sampler components for extraction as follows: <br /> a. Into a bottle, transfer the filter, front sorbent section and glass wool plugs. Add 10.0 mL <br /> toluene. After desorption is complete, dilute a 1-mL aliquot to 10 mL for analysis. <br /> b. Into a second bottle, transfer the stainless steel screen. Using a 10-mL volumetric pipet, <br /> rinse the inner surfaces of the cassette into the bottle with hexane. <br /> c. Into a scintillation vial, transfer the back sorbent section. Add 10.0 mL toluene. <br /> 7. Cap each container and allow to stand 30 min, with occasional swirling. <br /> NOTE: A suitable internal standard such as p,p'-DDT may be added, at 0.4 pg/mL, at this point. <br /> NIOSH Manual of Analytical Methods(NMAM), Fourth Edition, 8/15/94 <br />